Bacterial Toxin Research Citations

Experimental Induction of Autoimmune Encephalomyelitis With Intracranial or Intramuscular hPSC Delivery:

Mice were immunized by subcutaneous injection of 300 µg of myelin oligodendrocyte glycoprotein (MOG) peptide corresponding to amino acids 3555 (MOG3555), diluted in normal saline (0.9% NaCl), and emulsified with complete Freund’s adjuvant. Bordetella pertussis toxin (300 ng; List Biological Laboratories, Inc., Campbell, CA, http://www.listlabstage.wpengine.com) was diluted in 0.2 ml of normal saline and injected i.p. immediately after MOG immunization and 2 days later. Clinical signs of EAE typically appeared 1012 days after immunization, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…On the day of, and 2 d after, immunization, the mice were also inoculated i.p. with 200 ng of pertussis toxin (List Biological Laboratories). Adoptive transfer (passive) EAE was induced by injecting recipient mice with 5.0 106 polarized MOG-specific Th17 cells.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE

EAE was induced by a subcutaneous immunization with 200 μg of MOG peptide (35-55) (Sangon Biotech Co.) and 1 mg of a heat-killed H37Ra strain of Mycobacterium tuberculosis (Difo) emulsified in complete Freund’s adjuvant injected into the back and head region. This was followed by an injection of pertussis toxin (List Biological Laboratories) at a dose of 200 μg/mouse in the tail vein. Forty-eight hours later, pertussis toxin was administered again. Disease severity was scored as previously described 15.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…and pertussis toxin was purchased from List Biological Laboratories (St. Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin (PTX), List Biological Labs #180, Campbell, CA…

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE Induction:

EAE was induced as described …Two hundred nanograms of pertussis toxin (List Biological Laboratories, Inc) in 200µl PBS was injected intraperitoneally (i.p.) on the day of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

200 ng pertussis toxin (List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. Mice were examined daily and scored for disease severity using the standard scale…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (400 ng; List Biological Laboratories, Campbell, CA) was administered ip on days 0 and 2. CD4+ T cell-reconstituted Rag1 2/2 mice were immunized with 150 mg of MOG peptide emulsified in CFA at day 0 and day 7 as previously described (28). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Construction of NHEK cell sheets:

… Osaka, Japan), 10 ng/mL recombinant human epidermal growth factor (Protein Express, Chiba, Japan), 5 g/mL transferrin (Life Technologies), 5 mg/mL insulin (Gibco, Thermo Fisher, MA, USA), 0.4 mg/mL hydrocortisone (Wako), 1 nM cholera toxin (List Biological …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mouse model of -lactoglobulin-induced food allergy:

The mice were divided into four experimental groups, and each group consisted of 10 mice. Four groups of mice were treated as follows: (Group 1) the normal control group was fed normal saline (2 mL each time for 7 continuous days); (Group 2) the positive group (as shown in Figure Figure2A)2A) received the mixture of 20 mg -lactoglobulin (BLG) (Sigma, Buchs, Switzerland) and 10 µg CTX (Cholera toxin, List Biological Laboratories, Campbell, CA, United States) on days 0, 7, and 14 by intragastric gavage (2 mL of the mixture was used each time). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae