Bacterial Toxin Research Citations

… After acclimating to the environment for one week, mice were subcutaneously injected with MOG35-55 (200 µL, 1 mg/mL). Pertussis toxin (LOT: 180244A1; List Biological Laboratories, CA, USA) was dissolved in physiological saline (200 ng/100 µL). …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE induction and MOG restimulation

Mice used in each experiment were 4- to 12-month-old female littermates. Experimental autoimmune encephalomyelitis (EAE) was induced by subcutaneous injection of mice (four per group) with 200 μg myelin oligodendrocyte glycoprotein (MOG) peptides (amino acids 35–55: MEVGWYRSPFSRVVHLYRNGK; Genemed Synthesis)54 emulsified in CFA (Chondrex). Mice were also intraperitoneally injected with 200 ng pertussis toxin (List Biological Laboratories) on days 0, 1, and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

4.2. Mice and EAE induction

EAE was induced 4 weeks following vector administration in female C57BL/6 mice (The Jackson Laboratory, Bar Harbor, ME, USA). Briefly MOG35-55 (200ug) emulsified in Complete Freund’s Adjuvant (Sigma Aldrich, St. Louis, MO) with 9 mg/mL Mycobacterium tuberculosis (Difco Laboratories, Franklin Lakes, NJ) was injected subcutaneously over two sites at the upper and lower back. 2- and 24- hours following immunization, 200 ng Pertussis Toxin (List Biological Laboratories, Inc, Campbell, CA) was injected interperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In TeNT treatment, an 8-nl mixture containing 0.2M KCL, 0.5% dextran Antonia Red 150,000 MW (Sigma) and TeNT (List Labs, 190A; 0.5M) was pressure injected into the hindbrain ventricle. Between 50 and 70 min after injection, dextran was imaged, followed by another 10 min of GCaMP6s imaging. …

• Product #190 – Tetanus Toxin from Clostridium tetani

… Three days before euthanasia, rats were anesthetized with isoflurane (5% for induction; 2-2.5% for maintenance), and 15-20 µL cholera toxin B subunit (CTB, #104 List Biological Labs, Campbell, California, USA) was bilaterally injected into the intrapleural space to retrogradely label PhMNs, as previously described (Cheng et al 2021a) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis toxin (List Biological Laboratories) was administered intraperitoneally on days 0 and 1 postimmunization. Following an established scoring system, ocular disease progression was assessed using fundus examinations. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Enzyme-linked immunosorbent assay

Whole toxin A (List Biologicals, Campbell, CA) or recombinant rbdB was used to coat U-bottom high-binding 96-well ELISA plates (Greiner Bio-One, Frickenhausen, Germany). …

• Product #152C – Toxin A from Clostridium difficile

… The emulsion was injected subcutaneously into both hind limbs of each animal. The mice were treated intraperitoneally with 200 ng of pertussis toxin from Bordetella pertussis (dissolved in PBS; List Biological Lab, USA) at the time of immunization and 48 hours after …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… All animals received 2 subcutaneous injections of 100 µL each of MOG and a single intraperitoneal injection of 400 ng pertussis toxin (List Biological Laboratories, #180) in 200 µL of PBS. Mice received a second injection of PTx 48 hours after the initial injection. Mice were monitored and clinical scores were …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Induction of EAE

Active EAE was induced following previously published procedures [23]. In brief, 8–10-week-old female C57BL/6J mice (#000664, The Jackson Laboratory) were injected subcutaneously in both flanks with a total of 100 μg of MOG35-55 peptide (EZBiolab) in complete Freund’s adjuvant (CFA) containing 4 mg/mL Mycobacterium tuberculosis (DIFCO Laboratories). Mice also received 400 ng of pertussis toxin (LIST Biological Laboratories) intraperitoneally both immediately after immunization and 48 h later. Control, mock-injected mice were injected with everything except the MOG peptide. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer