Bacterial Toxin Research Citations

Induction of EAM and use of different adjuvants:

For the induction of EAM, we used the myocarditogenic peptide derived from the sequence of the murine cardiac myosin heavy chain (DSAF DVLS FTAE EKAG VYK for A/J mice and Ac-SLKLMATLFSTYASAD for BALB/c mice)… On day 0, all mice additionally received 500ng of pertussis toxin ip (List Biologicals, Campbell, CA). Individual experiments were conducted at least three times with five mice per group.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

Nave WT and CD73^-/- mice were immunized subcutaneously with 100g MOG(3555)…200 ng Pertussis toxin (List Biological Laboratories) was given intraperitoneally on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal models:

…Immunized mice also received 400ng of pertussis toxin (List Biological Laboratories, Campbell, CA) i.p. on days 0 and 2. The animals were scored daily for clinical signs of EAE.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Then, 80 µl of CTb neural tracer (1 mg/ml; List Biological Laboratories, Inc., Campbell, CA), in a solution containing 10% dimethylsulfoxide, was injected into the vitreous chamber of the left eye with a glass micropipette attached to a 100 µl Hamilton syringe. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

For retrograde tracing, …subunit B of cholera toxoid (CtB; 1% in distilled water; List Biological, Campbell, CA) or …were unilaterally injected into selected areas of the VTA or RMTg.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

– … THP-1 cells were differentiated with phorbol 12-myristate 13-acetate (50 ng/ml; Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight then treated with LT (List Biological Laboratories, Campbell, CA, USA) containing 100 ng/ml lethal factor and 200 ng/ml protective …

Lethal Toxin consists of Lethal Factor & Protective Antigen:

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence). 

• Product #171 (Anthrax Protective Antigen (PA), Recombinant from B. anthracis)

…Vero cells treated with purified C. difficile toxins (A and B) …(List Biological Labs, 152C and 155C;…) were used as controls. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

…Mealworm or shrimp extract in PBS (20 mg of protein) with 10 µg of cholera toxin (CT; List Biological Laboratories, Campbell, Calif) was administered by means of gavage. Control animals received PBS and CT only. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents and antimicrobial peptides:

…Lipopolysaccharide purified from E. coli 0111:B4 was purchased from List Biological Laboratories, Inc. …

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

… A set of CFA-control mice were also used as control for EAE induction. Two intraperitoneal injections of pertussis toxin, Bordatella pertussis, (List Biological Labs) were also administered to all mice on the day of induction and 48 hours thereafter. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)