Bacterial Toxin Research Citations

Retrograde and Fos double labeling of pre-oculomotor neurons:

In the last four rats, cholera toxin B (CTB) was delivered into the III to retrogradely label contralateral INC/DN pre-oculomotor neurons. …The negative control rats suffered stereotaxic frame mounting, craniotomy and saline injection alone without down-stretching the jaw^[18]. Then, coronal frozen sections (30 mm in thickness) were cut through the midbrain and pons. The sections were incubated with rabbit anti-Fos antibody (Santa Cruz Biotech) and goat anti-CTB serum (1:500, List Biological Laboratories, USA) overnight.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Detection of serum antibodies:

ELISA was used to detect and quantify antibodies binding Fha, LOS and PTx antigens from B. pertussis (List Labs, Campbell, CA, U.S.A.)

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

EAE induction, prevention, and treatment:

C57BL/6 mice (812 weeks old) were immunized subcutaneously with 6 mg/mL synthetic peptide of myelin oligodendrocyte glycoprotein (MOG₃₅₅₅) peptide (MEVGWYRSPFSRVVHLYRNGK,… In addition, the animals received 250 ng pertussis toxin (List Biological Laboratories) intraperitoneally (I.P.) on days of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

PBMC isolation and culture:

PBMC (peripheral blood mononuclear cells) were isolated from collected blood or leukapheresis by density gradient centrifugation, according to the manufacturers instructions. Cells were cryopreserved in liquid nitrogen suspended in FBS containing 10% (vol/vol) DMSO. For PBMCs in vitro expansion, 2 x 10⁶ cells/ml were cultured in the presence of 5 g/ml Tetanus Toxoid (TT) (List Biological Laboratories Inc., Campbell, CA)…

• Product #191 – Tetanus Toxoid from Clostridium tetani

…In the same buffer, 24.5 L of 6 M SNAPtide substrate (no. 521 from List Labs) was added to the enzyme + compound solution, and the plate was read for 25 min at 490 nm (ex)/523 nm (em)/495 nm (cutoff). …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

…a membrane permeable, nonhydrolyzable cAMP analog, and TeNT (20 nM; List Biological Laboratories), which cleaves the synaptic vesicle protein synaptobrevin and prevents transmitter release…

• Product #190 – Tetanus Toxin from Clostridium tetani

Materials:

Staphylococcus aureus enterotoxin B (SEB, List Biological laboratories, INC, CA) were utilized to induce nasal polyp in sino-nasal cavity.

Animal Nasal Polyp Model:

…We prepared 5 different treatment formulations: PBS vehicle without RSV, a bolus RSV solution, and 3 distinct suspensions of PLGA/PEG NM in PBS. The formulations were intranasally administered 1 day after the OVA and SEB administration, which were scheduled 3 times per week. At each administration, 20l of the same formulation was applied to both nostrils; hence, a total of 40l was administered to each mouse. …

…On day 1, each mouse was injected subcutaneously with 10 µl of MOG emulsion and injected intraperitoneally with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) diluted in sterile PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and immunizations:

TNP‐Ficoll (Biosearch Technologies; 10 μg/mouse in PBS) and NP‐CGG (Biosearch Technologies; 10 μg/mouse in PBS:Alum (Thermo Scientific) 3:1) immunizations were done by intra‐peritoneal (i.p.) injection. For cholera toxin (Ctx; List Biological Laboratories, Inc.) immunization, mice were starved for 2 h and HBSS + 7.5% sodium bicarbonate solution (4:1 ratio) was administered by intra‐gastric (i.g.) gavage, followed 30 min later by i.g. gavage of 2.5 μg active Ctx in PBS. Mice were culled at d14 post‐immunization for analysis. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

Along with immunization and on day 2 postimmunization, 200 ng of pertussis toxin (List Biological Laboratories) or …was administered by intraperitoneal injection, when indicated.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)