Bacterial Toxin Research Citations

Primary mouse macrophage culture and treatment:

WT and miR-223^-/- mice were obtained from Jackson Laboratories. …Treatments consisted of unstimulated controls, LPS (100 ng/ml, LCD25; List Biological Laboratories) or a combination of LPS (100 ng/mL) & IFN (100 ng/ml) for 24 hours.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Protein preparation and purication:

The PTx holotoxin (PTx)…were obtained from List Biological Laboratories.

PTx was used utilized in: PTx binding assays; Murine PTx leukocytosis study;  In vitro PTx neutralization assay

Author specified that List Labs Product #180 was utilized in their research.

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… to as OVA- A594) (Invitrogen, #O34783) was added to the wells at 5 g/ml formulated with the indicated TLR ligand as adjuvant, ie 100 ng/ml E. coli strain O111:B4 LPS (LIST Biological Laboratories, Page 14. 14 USA), …

• Product #201 – LPS from Escherichia coli O111:B4

Induction of experimental autoimmune encephalomyelitis

… Control animals were given CFA without MOG peptide. Two hours and 48 h after EAE induction, all animals received an i.p. injection of 0.3 µg of pertussis toxin (List Biological Laboratories, Campbell, CA, USA)in 100 µL of saline. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Pertussis toxin from Bordetella pertussis, pertussis toxin subunit (B-oligomer), and diphtheria toxin (DTx) from Corynebacterium diphtheria were from List Biological Laboratories Inc.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Iontophoretic tracer injections:

For retrograde tracing, we deposited 1% cholera toxin subunit B (CTB; List Biological Solutions) dissolved in distilled water into either the superior paraolivary nucleus (SPN) (n = 5), medial nucleus of the trapezoid body (MNTB) (n = 4), or lateral superior olive (LSO) (n = 3). One brain had tracer spread from the deposit to the SPN and MNTB, and was classified as such for quantification. In some animals…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…of the retrograde tracer cholera toxin subunit b – CTb (low salt, 1% in distilled water, 104, List Biological Laboratories, Campbell … CTb was detected using a polyclonal anti-CTb raised in goat (703, List Laboratories, Burlingame, CA; dilution 1:10,000), tyrosine …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE model of MS:

Female SJL/J mice (JAX Laboratory, No. 000686) at the age of 8 to 9 weeks were injected subcutaneously in each axillary areas with PLP 139151 peptide (100 µg/mouse), together with 400 µg Mycobacterium tuberculosis H37 RA …Pertussis toxin, 500 ng (List Biological Laboratories) was injected i.p. on the day of the immunization and on day 2 post-immunization. Mice were scored daily for clinical signs of EAE on a scale from 1 to 5 according to the severity of the disease. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Primary MTE cells were isolated from C57BL6/J mice or DUOX1-deficient mice (generated by Lexicon Pharmaceuticals; Ref. 71) as previously described (24) by overnight incubation of tracheas with 0.1% protease 14 (Sigma) and culture of MTE cells on rat tail collagen I gel (BD Biosciences) in DMEM/F-12 media (Invitrogen) supplemented with 20 ng/ml cholera toxin (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials and Methods:

… Pertussis toxin List Biological Laboratories, Campbell, USA. …

2.2.6 Animal experiments

2.2.6.1 Immunization with MOG35-55 peptide and treatment with LPS For the induction of EAE, MOG35-55 peptide mixed with CFA was used. Mice were anaesthetized using a combination narcotic of 8 % ketamine and 4 % xylazine hydrochloride, in sterile 0.9 % NaCl. The animals were injected with 100 µl of MOG35-55 immunization paste (50 % MOG35-55 peptide, 50 % CFA), mixed and incubated at 4°C for 90 min prior to injection) subcutaneously in two different sites. Two hundred µl of pertussis toxin solution were applied intraperitoneal (i.p.), and mice were observed while recovering from narcosis.

 
Forty-eight hours after immunization with MOG35-55 immunization paste, the animals were injected with a second bolus of 200 µl pertussis toxin solution. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)