Bacterial Toxin Research Citations

…pertussis toxin (PTX) from List Biological Laboratories (Campbell, CA, USA), and …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CT-ELISA and -galactosidase activity assay:

…Purified CT subunit B (List Biological Laboratories, Inc., Campbell, CA) was used to provide a standard curve, and phosphate-buffered saline (PBS) was used as a negative control. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Mice, cells and reagents:

…Salmonella minnesota ultra pure lipopolysaccharide (LPS) from List Biologicals Laboratories (Campbell, CA)…

Mass cytometry (CyTOF) analysis:

Blood was collected from healthy donors in heparin tubes and stimulated with LPS (100ng/ml) with or without propionate (2mM) and brefeldin A.

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

… littermates that successfully entered and survived status epilepticus were selected to receive either DT (List Biological Laboratories, Product No: 150, 40 g/kg/dose intraperitoneal) or saline treatment. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diptheria toxin (DT) (1.0mg) (List Biological Laboratories, Campbell, CA) was reconstituted with 1.0ml sterile distilled water and then diluted to 2ng/ml in sterile PBS. DT (20ng/g i.p) was administered to mice to induce monocyte/ M depletion in CD11bDTR transgenic mice and FVB strain controls 24h prior to experiments.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Synovial tissues and synovial fibroblast cell cultures:

…Cells were cultured in Dulbeccos modified Eagles medium supplemented with 10% fetal calf serum. SF of passages 48 were used for all experiments. RASF and OASF were stimulated or not for 24 hours with Poly(I:C) (10ug/ml; Invivogen), LPS (100ng/ml; List Biological Laboratories) or TNF (10 or 100ng/ml; R&D) alone or in combination with IL1 (1ng/ml; R&D).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

TeNT surgery:

For ex vivo modeling of chronic epilepsy, rats were injected with unilateral intrahippocampal TeNT (2.5 ng in 1 l PBS with 2% BSA; List Biological Laboratories Inc., California, USA; (Mellanby et al., 1977; Jiruska et al., 2010b). Stereotaxic injections were targeted to the CA3 region of the right ventral hippocampus (co-ordinates relative to bregma: AP 4.3 mm, ML +4.4 mm, DV 7.5 mm). …

• Product #190 – Tetanus Toxin from Clostridium tetani

… anthrax lethal factor (batch 1692A1B), and protective antigen (batch 17117A1B) from List Biological Laboratories. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

List Labs Product #169A (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Lyophilized) has been discontinued; however, Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Liquid) is available for purchase.

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

Experiment 3: Retrograde Tracing from the SCN to the Forebrain During WC. – Cholera toxin administration:

The hamsters were positioned in a stereotaxic frame with the incisor bar set to 2.0 mm below the interaural level. The scalp was incised and a burr hole drilled at 0.5 mm anterior, 1.25 mm lateral from bregma. A glass electrode was back filled with the subunit of cholera toxin (CT; 1% in distilled H₂O; List Biological Laboratories). A wire composed of 10% (wt/wt) iridium and platinum was placed in the CT and attached to the lead, …Once lowered, the CT iontophoresis took place using a positive current of 1.5 A in alternating 5 s on/5 s off pulses for 10-12 min. Following the iontophoresis, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE:

… In brief, B6 mice were s .c. immunized with 100 µg of MOG 35 55 peptide in complete Freund’s adjuvant containing 0.4 mg Mycobacterium tuberculosis H37RA (Difco, Lawrence, KS). Two hundred nanograms of pertussis toxin (List Biological Laboratories, Campbell, CA) was adminis tered i.v. on day 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)