Bacterial Toxin Research Citations

… Six-week-old female specific-pathogen-free BALB/c mice, purchased from Charles River Laboratories (Saint Germain sur l’Arbresle, France), were sensitized with 1 mg of either OVA, OM or LYS, plus 10 µg of cholera toxin (CT, List Biologicals, Campbell, USA),…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reaktivita imunitnho systmu byla podpoena intraperitoneln aplikac 0,6 a 1,2 g PT (pertusov toxin; List Biologicals, Campbell, USA), rozputnho v PBS (phosphate buffered saline).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Recombinant protective antigen (rPA) was purchased from List Laboratories. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

ELISA to assess cholera-specific IgG concentrations was performed by coating the plates with a final concentration of 1 µg/ ml with the Vibrio (V.) cholerae strain Inaba 569B (List Biological Laboratories) and incubated for 3 h at 37°C …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Staphylococcus aureus enterotoxin B (SEB, List Biological laboratories, INC, CA) were utilized to induce nasal polyp in sino-nasal cavity. …

EAE:

…0.2 ml inoculum containing 200 µg MOG peptide 3555 (MEVGWYRSPFSRVVHLYRNGK) in IFA (DifcoLabs, Detroit, MI) with 800 µg Mycobacterium tuberculosis hominis H37Ra (MT) on day 0 and 7 following [28], with pertussis toxin (PTx) (List Biologicals) 200 ng ip on …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

EAE was induced in female C57BL/6 mice (The Jackson Laboratory) at 812 weeks of age by subcutaneous immunization in the flank with an emulsion containing 200 µg of myelin oligodendrocyte glycoprotein peptide 3555 (MOG₃₅₅₅) in saline and an equal volume of complete Freund’s adjuvant (CFA) containing 400 ng of mycobacterium tuberculosis H37RA (Difco Laboratories, Detroit, MI, USA). All mice were administered 400 ng of pertussis toxin (List Biological, Campbell, CA, USA) intraperitoneally (i.p.) at 0 and 48 h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Craniotomies were made on both sides of the parietal bone, and a glass electrode (tip diameter: ~10 m) filled with 1% cholera toxin subunit B (CTb; List Biological Laboratories, Campbell, CA) dissolved in phosphate buffered saline (PBS) was inserted into the brain. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

At PND 5 rat pups were injected intraperitoneally (i.p.) with saline or LPS (2 mg/kg) dissolved in saline (LPS isolated from E. coli 055:B5, List Biological Laboratories, INC, Campbell, CA, USA).

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

…and Salmonella minnesota R595 LPS (List Biological Laboratories) were used as noted. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)