Citations

EAE:

… emulsified in complete Freund’s adjuvant (CFA). Pertussis toxin (List Biological laboratories, Inc.) 200 ng was injected intravenously on the day of immunization and 48 h later…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune uveoretinitis (EAU):

…On the day of immunization, mice also received an ip injection of 0.5 µg Bordetella pertussis toxin (List Biological Laboratories).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… We used Cholera toxin-B-biotin conjugate (List Biological Labs, Campbell, CA) as a probe for GM1 …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

Evocation of visual responses and deposition of neural tracers:

…The stereotaxic coordinates of visual responses were then used either in the same bird or different birds to guide glass micropipettes to visually responsive loci for the recording and deposition of neural tracers. The glass micropipette (WPI, 1.5 mm outside diameter were pulled in a David Kopf vertical puller (Model 700C) and broken back to produce tips of between 12 and 20 microns internal diameter. These were filled with either biotinylated dextran amine (BDA, Molecular Probes, either 10,000 or 3,000 molecular weight, 10% in phosphate buffered saline (PBS)) or cholera toxin B-chain (choleragenoid; List Laboratories, Campbell, CA, 1% in PBS). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Botulinum A toxin BoNTA, List Biological Laboratories, …

EAE Induction:

Experimental autoimmune encephalomyelitis was induced as previously described. Briefly, mice were immunized subcutaneously with 150µg of MOG₃₅₅₅ (Pepceuticals, UK) emulsified in CFA (BD Difco, Germany) and received 200ng pertussis toxin (PTx, List Biological Laboratories, Inc.) intraperitoneally at the time of immunization and 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

ELISPOT:

Peripheral blood mononuclear cells (PBMCs) were prepared and washed in RPMI medium supplemented with 10% fetal calf serum, penicillin, streptomycin, and L-glutamine. …The antigen, recombinant Protective Antigen (rPA), LF or EF (List Biological Laboratories Campbell, Calif.) was added at a final concentration of 12.5 g/ml for PA or 25 g/ml for either LF or EF. The plates were incubated overnight at 37C in a 5% CO₂, humidified atmosphere. …

Quantitative anti-PA IgG ELISA:

Wells of 96-well microtiter plates (Immulon 2HB; Dynex Technologies, Chantilly, Va.) were coated by adding of 100 l rPA (List Biological Laboratories Campbell, Calif.) diluted to 1 g/ml in PBS. Plates were incubated overnight at 4C. …

Lethal toxin neutralization assay (TNA):

…Lethal toxin (LeTx; 100 ng of rPA/ml and 50 ng of LF/ml, final concentrations) was prepared in the same medium and pre-incubated with the sample dilutions in a humidified incubator set at 37C, 5% CO2 for 1 h. LeTx was prepared using PA obtained from List Biological Laboratories …

End-point ELISA for LF and EF:

Wells of 96-well microtitre plates (Immulon 2HB) were coated with either LF or EF (List Biological Laboratories) at 1 g/ mL in PBS (100 L per well). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

 Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Cell Depletion:

KPCD mice received one intraperitoneal injection of DT (25ng/g body weight; List Biological Laboratories) or matched volume of PBS. Monocyte depletion was analysed 24h later by flow cytometry.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Motor and sensory neurons were retrogradely labeled in vivo at p14p16 by intramuscular injection of 1% unconjugated Cholera toxin B subunit (CTB, List Biologicals). Animals were analyzed after 57 days.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Chemicals and Reagents:

… Recombinant anthrax lethal factor (LF) from B. anthracis was from List Biological Laboratories (Campbell, California).

Characterisation of the thresholds for LF and EF detection:

In a first step, we characterised the threshold for the enzymatic quantification of LF and EF by using plasma and ear tissue samples derived from mice infected…

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).