Bacterial Toxin Research Citations

… Staphylococcus aureus enterotoxin B (SEB, List Biological laboratories, INC, CA) were utilized to induce nasal polyp in sino-nasal cavity. …

EAE:

…0.2 ml inoculum containing 200 µg MOG peptide 3555 (MEVGWYRSPFSRVVHLYRNGK) in IFA (DifcoLabs, Detroit, MI) with 800 µg Mycobacterium tuberculosis hominis H37Ra (MT) on day 0 and 7 following [28], with pertussis toxin (PTx) (List Biologicals) 200 ng ip on …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

EAE was induced in female C57BL/6 mice (The Jackson Laboratory) at 812 weeks of age by subcutaneous immunization in the flank with an emulsion containing 200 µg of myelin oligodendrocyte glycoprotein peptide 3555 (MOG₃₅₅₅) in saline and an equal volume of complete Freund’s adjuvant (CFA) containing 400 ng of mycobacterium tuberculosis H37RA (Difco Laboratories, Detroit, MI, USA). All mice were administered 400 ng of pertussis toxin (List Biological, Campbell, CA, USA) intraperitoneally (i.p.) at 0 and 48 h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Craniotomies were made on both sides of the parietal bone, and a glass electrode (tip diameter: ~10 m) filled with 1% cholera toxin subunit B (CTb; List Biological Laboratories, Campbell, CA) dissolved in phosphate buffered saline (PBS) was inserted into the brain. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

At PND 5 rat pups were injected intraperitoneally (i.p.) with saline or LPS (2 mg/kg) dissolved in saline (LPS isolated from E. coli 055:B5, List Biological Laboratories, INC, Campbell, CA, USA).

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

…and Salmonella minnesota R595 LPS (List Biological Laboratories) were used as noted. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Induction of relapsing EAE:

Female SJL/J mice (Charles River, Shizuoka, Japan), 810 weeks old, were immunized with the encephalitogenic proteolipid peptide (PLP)139-151 (sequence: HSLGKWLGHPDKF; purity>90%) emulsified in complete Freund’s adjuvant (Difco, Detroit, MI) plus Mycobacterium tuberculosis H37Ra (BD, Sparks, MD) (5 mg/ml). Then, mice were immunized with 0.2 ml of the emulsion injected subcutaneously into two locations at the tail root. At the time of immunization and 48 h later, the mice were administered 400 ng of pertussis toxin intravenously (List Biological Laboratories, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For other injections, cholera toxin B (List Biological Laboratories, Inc) or latex microspheres (Lumafluor … The CTB polyclonal antibody (List Biologicals, Cat# 703, Lot# 7032A8, AB_10013220) was generated against CTB in goat. …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

…Immediately and 24 h after immunization, mice were administered 200 ng pertussis toxin intraperitoneally (List Biological Labs). At 1 week after induction, EAE mice received a booster of 200 µg of MOG in incomplete Freund’s adjuvant. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Depletion:

For in vivo cell depletion, DT (0.5 mg/mouse/dose, List Biological Laboratories) was given intraperitoneally every 3 days for four times, then mice were sacrificed on day 10. In HFD-fed animals, DT was given in the last 10 days of the indicated HFD feeding period.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae