Bacterial Toxin Research Citations

C. difficile TcdA immunoblotting:

C. difficile TcdA (List Biologicals, Campbell, California, USA) binding to PSGL-1/mIgG2b was assessed by incubating the nitrocellulose membrane with 1 g/mL TcdA followed by anti-C. difficile Toxin A chicken IgY (List Biologicals) diluted 1:1,000 and…

• Product #152C – Toxin A from Clostridium difficile

Induction and treatment of EAE:

Mice were immunized subcutaneously with 300 µg MOG₃₅₅₅ peptide….Pertussis toxin (200ng, List Biological Laboratories, Campbell, CA, USA) in PBS was administered i.v. on days 0 and 2 post immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis (EAE):

Induction of EAE was performed according to the protocol of Miller and Karpus (2007) … Female mice, at 8-10 weeks old, were subcutaneously injected in two sites (100 µL by site) adjacent to the tail with the emulsion. Pertussis toxin (200ng) (List Biological Laboratories Inc., Campbell, CA, USA) was administered intraperitoneally on the same day of immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

SRF-RE luciferase assay:

For stimulated SRF response, HEK293 cells stably transduced with SyR-Gi were similarly transfected. The day after transfection, cells were serum starved overnight in the presence of 50 ng/ml pertussis toxin (List Biological Laboratories, Campbell, CA) and then stimulated for 6 h with CNO prior to harvesting the cells.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Sample preparation and analysis of RNA-Seq data:

…Cells were centrifuged (400g for 15 minutes at 4C), supernatant was collected, and BAL total cell counts and differentials were performed from cytospins. BAL samples from five of the healthy volunteers (samples 1115, Table 1) were cultured with RPMI1640 + 10% human AB serum and supplemented with 1% Antibiotic-Antimycotic (Life Technologies), with or without ultrapure LPS 100 ng/ml (E. coli 0111:B4, List Biological Laboratories, Cat# 421, Lot# 4217A1) at 37C and 5% CO₂ for 6 hours. Ultrapure LPS was chosen to minimize possibility of contamination by other bacterial components [1]. Previous studies have used concentrations of LPS ranging from 10 ng/ml-1000 ng/ml [1214]. The cell culture supernatants were collected and stored at -80C for later use. Total RNA was extracted by miRNeasy Mini Kit (Qiagen). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE induction:

… Pertussis toxin (List Biological Laboratories) was given intraperitoneally at 200ng/mouse on the day of immunization and 48h later. Clinical disease was assessed using the well-established EAE scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Six- to eight-week-old, sex-matched WT and mir17-92/ littermates were injected subcutaneously in the rear flank with 100 mg MOG3555 peptide (2HNMEVGWYRSPFSRVVHLYRNGK-COOH) in complete Freunds adjuvant (Sigma) on day 0, and 250 ng pertussis toxin (List Biological) was injected intraperitoneally on day 0 and day 1 after induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… tracings, we opened the skull above the accessible tectum, positioned the microelectrode onto the tectal lobe, and stereotaxically advanced to the deeper tectal layers where 100 nl of either a 1% solution of cholera toxin subunit B (CTb; v/v in PB, List Biological, Campbell, CA …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Pharmacokinetic and Pharmacodynamic Measurements:

… PA 83 (List Biologicals, Campbell, CA) immobilized on microtiter plates was used as a capture reagent. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Experimental SNAP-Etide assay:

Testing of compounds identified through the virtual screen was performed using a commercially available SNAP-Etide substrate (List Biological Inc., CA) as employed in prior work, …

• SNAP Etide Peptide Substrate (o-Abz/Dnp) for C. botulinum Type E Neurotoxin