Bacterial Toxin Research Citations

Immunofluorescent study:

Each section was pretreated in the same way as described above for immunohistochemistry. For the detection of GM1 ganglioside, biotinylated cholera toxin B subunit (1:500; List Biological Laboratories, Inc.) and …

• Product #112 – Cholera Toxin B Subunit Biotin Conjugate

… Alternatively, macrophages were stimulated using E. coli lipopolysaccharide (100 ng ml 1 ; Sigma-Aldrich, St Louis, MO, USA), Anthrax protective antigen and Anthrax lethal factor (List Biological Laboratories, Campbell, CA, USA), …

• Product #171 – Anthrax Protective Antigen (PA),Recombinant from B. anthracis
• Author did not specify which List Labs Lethal Factor was utilized.  List Labs offers Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Materials:

We purchased BoNT/A of 150 kDa molecular weight from List Biological (Campbell, CA, USA) in a formulation containing 80% saturated ammonium sulphate. …

Materials and Methods:

BoNT/A was radiolabelled by pretreatment with 2iminothiolane and incubation with 99mTc-gluconate. The labelled toxin 99mTc-BoNT/A was puried using size exclusion HPLC. Twenty-four female Wistar rats were evenly injected in the bladder wall with either 99mTc-/ (n = 12) or free 99mTc (n = 12). Four rats from each group were killed at 1, 3 and 6 h after injection, respectively. The bladder, L6-S1 spinal cord segment and L6-S1 dorsal root ganglia (DRG) were harvested and their radioactivity counted in a gamma scintillation detector. Results were calculated as % injected dose (I.D.) per gram of tissue. The paired t-test was used for comparison of means of 99mTc-/ radioactivity vs free 99mTc in the tissues of interest.

…All 45 mice were divided in five groups, with nine mice in each group. The first is the control group, which was exposed to 10 µg of cholera toxin (List Biological Laboratories, Inc., Campbell, CA, USA) only. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and analysis:

EAE was induced in 8 to 12 week-old mice by immunization with 100 µg MOG p35-55 or rhMOG in complete Freund’s adjuvant containing 200 µg Mycobacterium tuberculosis H37Ra (Difco Laboratories, Detroit, MI) on day 0. Mice received 200 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) IP on days 0 and 2. Mice were observed daily for clinical EAE.² Histology and immunohistochemistry analyses were performed as previously described.¹

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… Mice also received an intraperitoneal (i.p.) injection of Pertussis toxin (300ng in 200ul of PBS/ mouse) (List Biological Labs, Campbell, CA). Mice received this pertussis toxin injection again 48 hours 25 later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

AAV rat model:

Experimental AAV was induced in WKY rats as reported previously… The hMPO- and OVA-sensitized rats also received 800 ng of pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA) intraperitoneally on Days 0 and 2. All rats were sacrificed 8 weeks after hMPO immunization; blood, urine samples and tissues were obtained.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

…Pertussis toxin (PTX) was purchased from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… emulsified in complete Freund’s adjuvant (CFA). Pertussis toxin (List Biological laboratories, Inc.) 200 ng was injected intravenously on the day of immunization and 48 h later…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune uveoretinitis (EAU):

…On the day of immunization, mice also received an ip injection of 0.5 µg Bordetella pertussis toxin (List Biological Laboratories).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)