Bacterial Toxin Research Citations

EAE Induction:

Experimental autoimmune encephalomyelitis was induced as previously described. Briefly, mice were immunized subcutaneously with 150µg of MOG₃₅₅₅ (Pepceuticals, UK) emulsified in CFA (BD Difco, Germany) and received 200ng pertussis toxin (PTx, List Biological Laboratories, Inc.) intraperitoneally at the time of immunization and 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

ELISPOT:

Peripheral blood mononuclear cells (PBMCs) were prepared and washed in RPMI medium supplemented with 10% fetal calf serum, penicillin, streptomycin, and L-glutamine. …The antigen, recombinant Protective Antigen (rPA), LF or EF (List Biological Laboratories Campbell, Calif.) was added at a final concentration of 12.5 g/ml for PA or 25 g/ml for either LF or EF. The plates were incubated overnight at 37C in a 5% CO₂, humidified atmosphere. …

Quantitative anti-PA IgG ELISA:

Wells of 96-well microtiter plates (Immulon 2HB; Dynex Technologies, Chantilly, Va.) were coated by adding of 100 l rPA (List Biological Laboratories Campbell, Calif.) diluted to 1 g/ml in PBS. Plates were incubated overnight at 4C. …

Lethal toxin neutralization assay (TNA):

…Lethal toxin (LeTx; 100 ng of rPA/ml and 50 ng of LF/ml, final concentrations) was prepared in the same medium and pre-incubated with the sample dilutions in a humidified incubator set at 37C, 5% CO2 for 1 h. LeTx was prepared using PA obtained from List Biological Laboratories …

End-point ELISA for LF and EF:

Wells of 96-well microtitre plates (Immulon 2HB) were coated with either LF or EF (List Biological Laboratories) at 1 g/ mL in PBS (100 L per well). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

 Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Cell Depletion:

KPCD mice received one intraperitoneal injection of DT (25ng/g body weight; List Biological Laboratories) or matched volume of PBS. Monocyte depletion was analysed 24h later by flow cytometry.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Motor and sensory neurons were retrogradely labeled in vivo at p14p16 by intramuscular injection of 1% unconjugated Cholera toxin B subunit (CTB, List Biologicals). Animals were analyzed after 57 days.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Chemicals and Reagents:

… Recombinant anthrax lethal factor (LF) from B. anthracis was from List Biological Laboratories (Campbell, California).

Characterisation of the thresholds for LF and EF detection:

In a first step, we characterised the threshold for the enzymatic quantification of LF and EF by using plasma and ear tissue samples derived from mice infected…

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Induction of EAU and treatment:

Each C57BL/6J mouse was immunized subcutaneously in one rear footpad and the tail head, and rump (total of 200l) with an emulsion containing 300µg of human IRBP₁₂₀… Simultaneously, the mice received 1µg of Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitoneally. Then the mice were randomly divided into a chrysin-treated group (n=30 mice) and a control group (n=30 mice). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…immunized with 200 µg MOG 3355 peptide emulsified in complete Freund’s adjuvant (Sigma) containing Mycobacterium tuberculosis H37 RA (final concentration 2 mg/ml) on day 0, and ip administered with 200 ng pertussis toxin (List Biological Laboratories)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mucosal immunization

For intranasal (i.n.) administration, immunization cocktails were made by mixing the indicated proteins with PBS. Each dose (20 μl total, 10 μl per nostril) contained either CTB-MPR (35 μg ≡ 12 μg MPER ≡ 2 nmol MPER) with cholera toxin (CT, 1 μg, List Biological Laboratories), VLP preparation (1.1 mg total protein containing 48 μg dgp41 and 48 μg Gag, equivalent to 2 nmol and 1 nmol, respectively) with either CT (1 μg), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagent:  1 nM cholera toxin (List Biological Laboratory, Campbell, CA)

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and LPS instillation:

Active EAE was induced by immunizing the mice with MOG₃₅₅₅ peptide (100 µg) emulsified in complete Freunds adjuvant (Sigma) containing heat-killed Mycobacterium tuberculosis H37Ra (200 µg per mouse, DIFCO Laboratories) on day 0, and intraperitoneal injection of 200ng pertussis toxin (PTx; List Biological Laboratories) on days 0 and 2. To induce T_h17-mediated passive EAE, CD4⁺ T cells, obtained from 2D2 mice, were polarized with rIL-6 (20ng ml¹), rIL-23 (10ng ml¹), rTGF- (3ng ml¹) and anti-IFN- antibody (4 g ml¹) on a plate coated with anti-CD3 and anti-CD28 antibodies for 5 days and adoptively transferred to sublethally irradiated (450 rads) WT and Atg7 CKO mice. PTx (200ng) was intraperitoneally administrated on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)