Bacterial Toxin Research Citations

Induction of EAM:

… Mice were also injected with 500 ng of pertussis toxin ip on day 0 (List Biologicals). Induction of EAM by Adoptive Transfer of CD4+ T Cells …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Experimental autoimmune encephalomyelitis was induced as previously described… Bordetella pertussis Brain Struct Funct 123 toxin (250 ng; List Biological Laboratories, USA) was administered intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3. Induction of RR-EAE and in vivo treatments:

… Each mouse received s.c. injections of 200 µl emulsion, fractionated in two distinct sites draining axillary and inguinal lymph nodes. Pertussis toxin (PTX, 200 ng/mouse; List Biological Laboratories) was administered i.p. on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis:

…An intraperitoneal injection of 300 ng Pertussis toxin (List Biological Laboratories, Campbell, CA) was administered at the time of induction and 48 hours later. Control animals were treated with CFA (1.5 mg/mL) and Pertussis toxin alone.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

For active EAE induction, age- and sex-matched mice were immunized s.c. with MOG_35-55 peptide (300µg) mixed in CFA (Sigma-Aldrich) containing 5 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (Difco). Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Polyclonal CD4⁺ T cell adoptive transfer (in mice):

Foxp3-expressing T_reg cells were ablated following adoptive transfer by the i.p. injection of diphtheria toxin (DT; List Biological, Campbell, CA) at a concentration of 0.5 g/mouse on day 1 after adoptive transfer of T cells, and then 0.2 g/mouse every other until day 21.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Conjugation of Dylight 594 to CTXB:

For GM1 ganglioside detection, Dylight 594 (Thermo Cat #46412) was conjugated to the primary amines of Cholera Toxin B protein (CTXB) (List Biologicals Cat #104) following manufactures protocol. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

CT ELISA Assays:

CT production was measured using V. cholerae culture supernatants by GM1-enzyme-linked immunosorbent assays (GM1ELISA) as described previously [39]. Purified CT subunit B (List Biological Laboratories Inc., Campbell, CA, USA) was used to provide a standard curve and phosphate-buffered saline (PBS) was used as a negative control. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Stereotaxic surgery:

Ten 49118 days old male C57BL/6 J wild-type mice were anesthetized with isoflurane followed by an intraperitoneal injection of an anesthetic mixture. …In these cases, following FG injections, we injected the retrograde tracer 0.5 % Cholera toxin B subunit (CTB, List Biologicals, Campbell, CA, USA) into the HIPP of the left hemisphere at three coordinates: 2.5, 2.8, 3.2 mm posterior from bregma, 1.6, 1.6, 1.7 mm lateral from midline and 2.1, 2.1, 2.2 mm below the zero level, respectively. In the other four mice, FG was injected bilaterally into the PFC at the coordinates given above, and CTB was injected into the MS at the coordinates 1.0 mm anterior from bregma, in the midline and 4.1 mm below the zero level. We injected 1418 nl of FG and 1823 nl of CTB into every single injection spot using the Nanoject-R pump. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… weeks. Four days before sacrifice, 2 µL of 1 % cholera toxin B (CT-B; List Biologicals) was injected in the left sciatic nerve. Fifty-micrometer sagittal free floating sections were immunostained for CT-B (List Biologicals, 1:30,000). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt