Bacterial Toxin Research Citations

Tracer injections and tissue processing:

Mice were anesthetized with ketamine hydrochloride (100 mg/kg) and xylazine (3 mg/kg) intraperitoneally and placed in a stereotaxic apparatus. … cholera toxin B (List Biological Laboratories, Inc) were injected into the inferior colliculus, auditory cortex or amygdala using iontophoresis …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunization:

Immunizations consisted of …mixed with 5 µg LPS (List Biologicals) diluted in 1x PBS to a total volume of 100 µL per injection. Injections were administered retro-orbitally under isoflurane anesthesia.

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

EAE Induction:

EAE was induced using an emulsion of Freunds adjuvant plus Mycobacterium tuberculosis H37Ra (4 mg/mL) and either 200 µg MOG_35-55, or 50 µg PLP_179-191 diluted in phosphate buffered saline (PBS) [17]. Mice were then anesthetized using isoflurane and 200 µL of emulsion was administered subcutaneously dispersed over three locations on the back of the animal. 200 ng of pertussis toxin (List Biological Laboratories) diluted in PBS was administered retro-orbitally immediately following injection of the emulsion and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Lipopolysaccharid ( LPS ) , List Biologicals, Campbell, CA, USA Escherichia coli K12, D31m4 (Re) …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

 The ALF-type particles utilized in the original studies with A1-15 peptide contained native MPLA derived from gram-negative bacterial lipopolysaccharide as supplied by List Biological Laboratories (Campbell, CA USA), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Disease induction and EAE analysis:

EAE was induced in female C57BL/6 mice (Jackson Laboratories, Bar Habour, Maine), aged 8–9 weeks, by injecting subcutaneously (s.c.) 50μg of myelin oligodendrocyte glycoprotein peptide (MOG)35–55 in Complete Freund’s Adjuvant (CFA) (Fisher, Michigan USA) on day 0 [24–25]. Intraperitoneal (i.p.) pertussis toxin (0.1μg/200μl, List Biological labs, Hornby, ON) was administered on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vitro culture of T cells:

Antigen-presenting spleen cells or DCs were pulsed with 1gml¹ staphylococcus enterotoxin B (SEB; List Biological Laboratories)…

Animals, antigens and adjuvants:

Six-week-old female and male C3H/HeJ mice were purchased from the Jackson Laboratory (Bar Harbor, ME). BALB/c female mice (58 weeks old) were obtained from Taconic Farms (Hudson, NY). … Cholera toxin (CT) was purchased from List Biological Laboratories Inc (Campbell, CA).

Sensitization, allergen challenge and assessment of anaphylaxis:

Five-week old C3H/HeJ offspring were sensitized with CPE+CT by gavage (5 mg CPE + 20 µg CT/mouse) at weekly intervals for 6 weeks followed by 2 boosters at 2-week intervals (50 mg CPE + 20 µg CT/mouse), as previously published…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cholera toxin (azide-free) from Vibrio cholerae used for cAMP experiments was purchased from List Biological Laboratories (Campbell, CA) (catalog no. 100B).

Measurement of cAMP:

…Cells were incubated with forskolin (10 M), VIP (1 M), or cholera holotoxin (0, 1, or 100 nM) in culture media with 5% CO₂ at 37°C for 1hr, followed 2 washes with cold PBS, then lysed and flash frozen in liquid N₂…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemical Methods:

Immunocytochemical detection was performed by standard light, fluorescent microscopy…Rats were perfused transcardially (4% paraformaldehyde solution). Sections were incubated (overnight/4C)…goat anti CTb primary antibody (1/10,000; List Biological Laboratories).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction and disease scores:

EAE was induced in C57BL/6 (The Jackson Laboratory, Bar Harbor, ME) female mice (8-10 weeks old). …Pertussis toxin (List Biological Labs Inc., Campbell, CA) was administered by intraperitoneal (ip) injection at day 0 (75 ng per mouse) and day 2 (200 ng per mouse) after immunization. Mice were scored daily for EAE …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)