Bacterial Toxin Research Citations

Experimental autoimmune encephalomyelitis:

… For active EAE, Sort1 / and WT littermate mice were immunized sc with 250 µg MOG 3555 or whole-protein MOG…and received 200 ng pertussis toxin (List Biological Laboratories) ip at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracers and coordinates:

Retrograde tracing was accomplished using 3-10nl injections of Cholera toxin subunit B (CTb) (10 µg/µl saline, List Biological, Campbell, CA), whereas anterograde tracing was done using either 3-15 nl injections of biotinylated dextran amine (BDA) …

<• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Recovery Surgical Procedures: Retrograde Tracer Injections and Catheters (Studies 2 and 3):

…In rats to be used in study 2, one of the following retrograde tracers was microinjected into the left dorsal lateral column containing preganglionic sympathetic fibers: Fluoro-Gold (FG; 2% in deionized water; Fluorochrome, Denver, CO; n = 2), cholera toxin subunit (CTB; 1% in deionized water; List Biological Laboratories, Campbell, CA; n = 7), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… For optimum visualization of CTb, sections were incubated overnight at RT with a goat anti-CTb antibody (1:10,000 List Biological Laboratories, CA, USA) diluted in 0.1 M PBS containing 0.3 % Triton X-100 and 0.5 % BSA. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of EAE:

Induction of myelin oligodendrocyte glycoprotein (MOG) EAE was done as previously described.30,31 Shortly, 6- to 8-week-old female C57BL/6 mice were immunized with an emulsion containing 200 µg of MOG_3555…The inoculum (0.2 mL) was injected subcutaneously into right and left flanks. One hundred nanograms of pertussis toxin (List Biological Labs, Campbell, CA, USA) in 0.1 mL saline was also injected intraperitoneally on day 0 and 48 hours later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Analysis of TMV-PA Antigens for Reactivity against PA Specific Antibodies:

SDS-PAGE, ELISA and Western Blots – 
 
… For westerns, gels were transferred onto 0.2 um nitrocellulose
membranes (7 cm x 8.5 cm) in Tris-Glycine buffer (25 mM Tris, 192 mM Glycine, 20%
Methanol, pH 8.3) for 1 hour at 100V. Membranes were blocked with 2.5% non-fat milk
in TBS (20 mM Tris, 150 mM NaCl, pH 7.6) for 1 hour at room temperature. Primary
antibody (Goat anti-PA polyclonal, List Biologics Lot # 7712A2 …

… (c) Wash sections with 0.01 M PB three times for 5 min each time. (d) Bathe sections in primary anti-CTB antibody, for example, List (List Biological Lab) goat anti-CTB (1:5005,000) in 0.01 M PB containing 1 % BSA and 1 % TX-100 either overnight at 4 C or 35 h at RT. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Induction of Active EAE in Mice by Immunization with MOG and Adjuvant:

EAE was induced in female wild-type C57BL/6 mice or MT and IL-10deficient mice on C57BL/6 background…All mice were given 400 ng of pertussis toxin (List Biological) intraperitoneally at 0- and 48-h postimmunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

68-week-old female C57BL/6 mice were immunized subcutaneously on day 0 with 75 µg/mouse of MOG3555 peptide emulsified in complete Freund’s adjuvant supplemented with 400 mg/ml Mycobacterium tuberculosis H37Ra (Difco). Each mouse was subsequently administered 250 ng pertussis toxin (List Biological Laboratories) intraperitoneal on days 0 and 2 after MOG₃₅₅₅ peptide immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Synthesis of cholera toxin-based vaccine

Cholera toxin (CT) was obtained from List Biologicals Laboratories (USA) …

Immunizations and injections of antibodies

C57BL/6 mice synchronized by Depoprovera® treatment were immunized under anesthesia by vaginal application of CTBOVA (4μg) + CT (1μg) in PBS at days 0, 14, and 21. NKp46+ ILC cells were depleted after intraperitoneal (i.p) injection of 200μL of anti-NK1.1 mAb (PK136 clone, 1/20 dilution of ascite fluid) at days -9 and 2 before each immunization. Such protocol insured at least a 98% depletion of NKp46+CD3- ILC cells in spleen, genital lymph nodes and vagina without affecting invariant natural killer T (iNKT) cells. NKG2D/NKG2D ligands blockade was performed by i.p injection of 500μg of mAb anti-NKG2D (CX5) at days -1 and +1 before and after each immunization. For dendritic cells tracking experiments, mice were immunized once with CTBA488 (40μg) and CT (1μg) and were sacrificed after 7 days.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae