Citations

Induction, clinical evaluation, and treatment protocols of EAE:

EAE was induced by MOG₃₅₅₅ in female mice used between 8 and 10 weeks of age. …and then injected to the caudal vein with 200 ng of pertussis toxin (dissolved in 200 µl PBS, List Biological Laboratories, USA) at the time of immunization and 2 days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals/Reagents:

PTX was purchased from List Biological Laboratories (Campbell, CA, USA).

To determine whether Ca²⁺ flux-independent synaptic loss depends on G protein signaling, we treated slices with oligomeric A and pertussis toxin (PTX), an inhibitor of the heterotrimeric G_i/o protein family, at concentrations described before in slice cultures.²⁶ PTX administration did not prevent spine loss caused by A…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE was induced using standard protocols as described … Pertussis toxin (PT) (300 ng) (List Biological laboratories Inc., Campbell CA, USA) was injected intraperitoneally (pH=7, in saline) on day 0 and day 2. MOG71-90 and MOG101-120 were not sufficient to induce EAE in any genotype. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and Reagents:

… The cross-reactive material (CRM197), which is a nontoxic mutant of diphtheria toxin, was purchased from List Biologicals Laboratories, Inc. (Campbell, CA).

Conjugation of 1 with TT and CRM₁₉₇ carrier proteins:

The thiolated 1 was conjugated to the TT and CRM₁₉₇ carrier proteins in a two-step reaction using the NHS-(PEG)₂-maleimide crosslinker [(SM-(PEG)₂]. …

Conjugates with varying hapten densities were obtained by treating the TT and CRM₁₉₇ carrier proteins with an increasing molar conjugation ratio of the (SM-(PEG)₂ crosslinker to the carrier protein. The TT and CRM₁₉₇ carrier proteins were dialyzed overnight against PBS, pH 7.2. Aliquots containing 2 mg of the protein were mixed with increasing excess molar ratios (5, 25, 100, 200, 400 and 800) of the SM-(PEG)₂ crosslinker (250 mM stock in DMSO) and incubated for 2 h at RT. …

• Product #149A – Diphtheria Toxin CRM₁₉₇, Mutant

Induction of EAE:

EAE was induced using standard protocols as previously described [24, 34, 35]. Briefly, 810 week old female WT and cathepsin B, S, L or B and S deficient mice were anesthetised with ketamine-xylazine. These mice were subsequently injected subcutaneously with 200 µl of an emulsion of 50 µg MOG35-55 in complete Freunds adjuvant (0.5 mg/ml M. butyricum in paraffin oil) (CFA) (BD Difco, Franklin Lakes NJ, USA). Pertussis toxin (PT) (300 ng) (List Biological laboratories Inc., Campbell CA, USA) was injected intraperitoneally (I.P.) (pH = 7, in saline) on days 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Culture media and supplements:

… The culture medium was supplemented with 250 ng/ml amphotericin B solution (HyClone), 20 ng/ml cholera toxin (List Biological),…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Methods:

… Adult male SpragueDawley rats (290360 g; Harlan) under anesthesia (1 ml/kg body weight of 50 mg/ml ketamine and 10 mg/ml xylazine, intramuscular) received single, iontophoretic injections of a mixture of 2.5% P. vulgaris leucoagglutinin (PHAL; Vector Labs) and 0.25% cholera toxin B subunit (CTB; List Labs) targeted stereotaxically to the LHAjv region (Swanson, 2004). … For IHC detection of tracers, the sections were incubated in primary antibodies directed against either CTB (1:10,000; goat, List BioLabs) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… An incision was made in the neck, and the Xth cranial nerve was isolated from the carotid artery and injected 2 cm distal from the head one to two times with the neuronal tract tracer cholera toxin B (CTB, 1% in dH 2 O, … List Biological Laboratories, Campbell, CA, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of diarrhea:

Cholera diarrhea was induced by oral administration of 25 µg cholera toxin (List Biological Laboratories, Campbell, CA)⁴⁴. Fifteen hours later, we examined the water volume in the intestinal lumen.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction and Evaluation of EAU:

Lrrk2 (-/-) mice and their WT controls were immunized with bovine IRBP (150 g) and human IRBP peptide 120 (200 µg), emulsified with complete Freunds adjuvant (CFA) containing 2.5 mg/ml killed Mycobacterium tuberculosis (DIFCO, Detroit, MI), administered subcutaneously in a volume of 0.2 ml. In addition, the mice were injected intraperitoneally with 0.2 µg pertussis toxin (List Biological Laboratories, Inc., Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)