Bacterial Toxin Research Citations

Antibodies and Ligands:

…Cholera toxin B-subunit was from List Labs. …

Results – Statins alter intracellular cholesterol and GCS distribution:

In addition, 3-PEHPC mimicked rosuvastatin to disperse the elevated TGN cholesterol pool and prevent the Golgi/ER targeting of internalized Verotoxin or Cholera toxin B-subunits (Figure 7A). In control cells, VT-B and CT-B were rapidly internalized to the Golgi. Though some double-stained cells were evident, most cells bound either VT-B or CT-B, consistent with previously reported cell-cycle-dependent receptor Gb₃ and GM1 expression (Majoul et al. 2002). In double-labeled ACHN cells, VT-B and CT-B are co-localized. In statin or 3-PEHPC-treated cells however, the distinct TGN cholesterol pool is diminished and VT-B/CT-B are detected more diffusely throughout the cell (Figure 7A). These changes were not observed in cells treated with FTI-277 or GGTI-2133. Multiple Rab GTPases have been implicated in this GSL-mediated transport route…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

OT Induction in a Mouse Model of Orally Induced CMA:

Upon arrival, mice were fed the control or the FF/Bb diet ad libitum for a period of 9 days. During the OT phase, they received daily oral gavages with 0.5 mL PBS, PepMix (total 0.16 mg peptides/0.5 mL PBS; 0.04 mg of each BLG-derived peptide) or whole whey protein as a positive control for maximum OT induction (50 mg/0.5 mL PBS; DMV International, Veghel, The Netherlands) for a period of 6 days. In the first study (Figure 1), following the OT phase, all mice were fed the control diet and orally sensitized for five consecutive weeks with 10 µg cholera toxin (List Biological Laboratories, Inc., CA, USA) as an adjuvant with or without 0.5 mL homogenized whey (40 mg whey/mL PBS). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

We immunized mice of either gender with myelin oligodendrocyte glycoprotein peptide, amino acids 3555 (MOG₃₅₅₅, 100 µg per mouse, Mimotopes) emulsified in complete Freund’s adjuvant containing heat-inactivated mycobacterium tuberculosis H37RA (8 mg/ml, BD Biosciences), by injecting 25 µl emulsion subcutaneously at each of four sites over the shoulder blades and flanks followed by pertussis toxin (300 ng i.p., List Biological) on 0 and 2 d postimmunization (dpi).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracer injections:

At the conclusion of the experiment, the retrograde tracers cholera toxin subunit B (CTB; List Biological, Campbell, CA; 12%) and Fast Blue…were injected into area 11 and into area 13, respectively, in one monkey. The reverse was done in a second monkey; CTB was injected into area 13 and Fast Blue into area 11. Both sets of tracer injections were performed unilaterally.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… retrograde Choleratoxin subunit B (CTB) (List Biological Laboratories, CA, USA) … were iontophoretic injected in either DRN …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Pertussis toxin, lyophilized in buffer (List Biological Laboratories Inc.). Resuspend in 1 ml of sterile dH 2O for a concentration of 50 µg/ml and store at 4°C ( see Note 7). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin from Bordetella pertussis (List Biological Labs). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Histochemistry and immunohistochemistry:

Rats were transcardially perfused with room-temperature 0.1 M sodium phosphate buffer, pH 7.3, followed by ice-cold 4% paraformaldehyde in phosphate buffer. Brains were postfixed… Conventional or immunofluorescence protocols were used to reveal the localization of anterograde/retrograde tracers or proteins of interest, following previously described methods (Deutch et al., 1996). For animals receiving nonconjugated CTb injections, a goat anti-CTb antibody (List Biological, Campbell, CA; RRID:AB_10013220) was used.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… In addition, animals received 300 ng of Pertussis toxin by intraperitoneal (ip) route (List Biological Laboratories, Inc., Campbell, CA, USA) on days 0 and 2 post-immunization. Non-immunized (nave) animals were used as control group. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antibodies, reagents and medium:

… CT was from List Biological Labs (Campbell, CA, USA). …

In vivo sensitization, and BLG specific challenge

Mice were sensitized 5 times 1 week apart by oral administration of 10 µg of CT in association with 10 mg of antigen in a solution containing 0.2 M of NaHCO3, pH 9. An oral antigen challenge was performed 1 week after the last sensitization by gavaging 100 mg BLG….

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae