Bacterial Toxin Research Citations

Immunization with whole-cell vaccine:

Animals were intranasally immunized twice (with a week apart) with the whole-cell antigen (WCA) derived from strain RM200 (38). The dose of 10-l vaccine volume was made of 100 µg (dry weight) of WCA and 1 µg cholera toxin (CT) (List Biological Laboratories, Campbell, CA) as a mucosal adjuvant in PBS.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction:

7-week-old female C57BL/6 mice were purchased from DBL. EAE was induced by subcutaneous immunization with 100 µg of MOG₃₅₅₅ peptide…At 0h and 48h after immunization, mice were intraperitoneally treated with 200ng of pertussis toxin (List Biological Laboratories Inc.).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… LPS was from List Biological Laboratories Inc (Campbell, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

…Pertussis toxin was from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis:

… Mice received 100 ng pertussis toxin (List Biological Laboratories) i.p. on day 0….

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

DC Depletion with Diphtheria Toxin:

DC Depletion with Diphtheria Toxin CD11c-DTR and Mgl2-DTR mice were i.p. injected with 1 m g of diphtheria toxin (List Biological Laboratories) 1 day before infection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Vaccine Antigens and Adjuvants:

…Various adjuvants were used including cholera toxin (CT; List Biological Laboratories, Campbell, CA), …

Immunization

… For conjunctival immunization, 100 μg of ovalbumin (OVA) (Sigma-Aldrich, St. Louis, MO) and various adjuvants (0.1 μg to 10 μg poly(I:C) or 10 μg MPLA or 2 μg CT or 1 mg Imject Alum) were suspended in 5 μl of phosphate-buffered saline (PBS) and dropped weekly for 3 consecutive weeks onto both conjunctival sacs by a micropipette. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Murine EAE Experiments:

Female SJL/J mice (8-12 weeks of age) were immunized subcutaneously in the hind flanks with 50 µg of proteolipid protein(139151) emulsified in complete Freund’s adjuvant containing 0.5 mg/ml Mycobacterium butyricum… On days 0 and 2, 300 ng of pertussis toxin (List Biological Laboratories) in 250 µl of endotoxin-free PBS was injected intravenously.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Infections and EAE induction:

… Mice also received two i.p. injections with 200ng of pertussis toxin (List Biologicals) at time of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Injection of tracer:

To retrogradely trace projection neurons, animals were first anesthetized using 5 % isoflurane in oxygen, placed in a stereotaxic apparatus …through the dura mater, to the stereotaxic position of the parabrachial nucleus and 2 L of 1.0 % solution of cholera toxin subunit B (CTb) (List, Campbell, CA, USA) was slowly injected over a period of 20 min. To minimize leakage of the tracer, a 10 min waiting period was imposed before the micropipette was retracted from its position. CTb was injected 7 days prior to sacrificing the animals.

Immunohistochemistry:

… To examine lamina I projection neurons, horizontal sections were incubated in 10 % normal donkey serum (Jackson, West Grove, PA, USA) in PBS + T for 1 h at room temperature to block unspecific staining. Subsequently, spinal cord sections were incubated, for 48 h at 4 C, with the primary antibodies: goat anti-CTb at 1:5000 dilution (List Biological),

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)