Bacterial Toxin Research Citations

Drugs:

…Pertussis toxin was from List biological laboratories Inc. (Campbell, CA 95008, US). …

Results – Effect of Pertussis-toxin treatment:

In order to elucidate the identity of the endogenous G subunits mediating the NOP/G₁ interaction, and to evaluate their potential effects on NOP mediated -arrestin 2 recruitment, HEK293 cells stably expressing the human NOP (NOP/RLuc) receptor and either the G₁ subunit (G₁/RGFP) or the -arrestin 2 (-arrestin 2/RGFP) were treated for 20 h with 10 ng PTX. As shown in Fig 2, right panel, PTX treatment abolished the ability of the agonist to stimulate NOP/G-protein interaction, but had negligible effects on the stimulation of NOP/-arrestin 2 interactions…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell culture, transfection and selection

The Breast cancer cell lines MDA-MB-231 and MCF-7 (ATCC) were maintained in DMEM (Sigma) supplemented with fetal bovine serum (FBS, Invitrogen) and antibiotics and antimycotics (ABM, Invitrogen). Normal breast cell lines: MCF-12A and MCF10A (ATCC) were cultured in universal medium composed of DMEM/F12 medium supplemented with 10 μg/mL insulin, 20 ng/mL epidermal growth factor (EGF), 500 ng/mL hydrocortisone (all from Sigma) and 100 ng/mL cholera Toxin (LIST Biological Laboratories, Surrey, United Kingdom), 5 % horse serum (Invitrogen) and 1 % ABM. All established cell lines were used within 6 months of purchase from ATCC. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture:

Keratinocyte culture medium (KCM) was composed of a basal mixture of 3 parts DMEM to 1 part nutrient mixture F-12 Ham ), …1 nM cholera toxin (List Biological Labs, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Recombinant proteins and reagents:

Protective antigen (PA) and edema factor (EF) were purchased from List Biological. …

Cell seeding and incubation with toxins:  

For toxin treatment, patterned and nonpatterned peritoneal macrophages and BMDMs were incubated with PA at 1 g/ml and EF at 100 ng/ml for 6 or 16 h at 37C in a 5% CO₂ atmosphere. For some experiments, cells were incubated with PA alone at 1 g/ml.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

… Two adjuvant molecules were used: poly (I:C) (Invivogen, San Diego, CA) administered via the intravenous route (25 µg per mouse) and cholera toxin (CT, List Biological Laboratories, Campbell, CA) injected via the intradermal route (1 µg per mouse). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.1. Materials:

… Cholera toxin (CT) was from List Biological Laboratories, Inc. (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… In the experiments with LPS, animals (n = 36) received intraperitoneal (ip) saline-based injections of ultra-pure LPS (lot # 4231A1; List Biological Laboratories, Campbell, CA, USA) in the dose of 1 mg kg 1 ….

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Treatment conditions:

… To determine the effect of MPLA exposure on LPS-mediated cytokine activation, HUVECs were exposed to 10 g/ml of MPLA and co-incubated in fresh media containing 0% or 2% FBS with Ultra-Pure LPS from E. coli 0111:B4 (100 ng/ml; List Biological Laboratories, Inc. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Reagents:

Ultrapurified Escherichia coli (E. coli) 0111:B4 LPS was purchased from List Biologicals Laboratory, Inc. (Campbell, CA). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

…Pertussis toxin (300 ng in PBS; List Biological, USA) was injected intraperitoneally at the time of induction and a second dose was administered three days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)