Bacterial Toxin Research Citations

Animal Models (Cell depletion):

The CD11b⁺ cells were depleted in CD11b-diphtheria toxin receptor (DTR) mice (congenic in the C57BL/6 background) by intraperitoneal injection with diphtheria toxin (DT; 7.5 ng/g body weight; List Biological Laboratories) or phosphate-buffered saline (PBS) on days 7 and 9, with sacrifice on day 10. We gave DT twice as continued DT treatment is toxic and can lead to saturation of the DTR. Effects of CD11b⁺ cell depletion were assessed on day 8 and day 10 (1 day after treatment) as CD11b⁺ cells repopulate the liver 48 hours after DT depletion.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

T cell activation for phenotyping experiments:

… As a positive control, PBMCs also stimulated with staphylococcal enterotoxin B (List Biologicals) at 5 µg/ml.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials:

The HEK293S GnTI cell line was obtained from ATCC. LPS derived from Salmonella minnesota R595 (Re) was purchased from List Biological Laboratories, Inc. (Campbell, CA) and used for the factor C or factor B activation assays and as free LPS to evaluate binding to LPS-immobilized Sepharose. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Macrophage depletion in diabetic CD11b-DTR mice restores PEDF protein expression to normal levels:

Male 6-wk-old B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J (CD11b-DTR) mice (stock no. 006000, Jackson Laboratory, Bar Harbor, MN) were given multiple intraperitoneal injections of vehicle or STZ. A diphtheria toxin (DT) (List Biological Laboratories) was injected intraperitoneally at the dose of 25 ng/g body wt weekly for 6 wk after STZ injection [25]. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

 In vitro Lipopolysaccharide and Q-VD-OPh Stimulation:

After 24 h, the cell culture medium was replaced with DMEM/ antibiotics. WT and caspase-6 KO primary microglial cells were stimulated with 10 ng/ml (diluted in sterile water) of Escherichia coli lipopolysaccharide (LPS; 055:B5; List Biological Laboratories, USA) or sterile water (control) each 12-well plate always contained both treatments. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

… Goat anti cholera toxin B-subunit (anti-CTb), List Biological Laboratories, Inc. … Cholera toxin subunit B [CTbList Biological Laboratories, Campbell, CA, USA] diluted in distilled water [1%] was used for the retrograde tracer injection control experiments. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

Isolation and culture of human urothelial cells

… Isolated cells were washed in keratinocyte serum-free medium (KSFM; Invitrogen) supplemented with 50 µg/ml bovine pituitary extract, 5 ng/ml human recombinant epidermal growth factor (both Invitrogen), and 30 ng/ml cholera toxin (List Biological Laboratories). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of EAE and Evaluation of Clinical Disease:

EAE was induced in mice as previously described… Subsequent to immunization, the mice received intraperitoneal injections of pertussis toxin (500 ng; List Biological Laboratories, Campbell, CA, USA) on the first day of sensitization and again after 2 d.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Recombinant protective antigen of anthrax (PA) and recombinant lethal factor (LF) were purchased from List Biologicals (Campbell, CA, USA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis