Bacterial Toxin Research Citations

Immunohistochemistry:

…To identify PMNs, four untreated rats received intrapleural injections of the retrograde tracer cholera toxin B fragment (CtB; List Biological Laboratories, Campbell, CA; Mantilla et al. 2009). Briefly, each rat was anesthetized with isoflurane (11.5% in 100% O₂), and a 50-l Hamilton syringe with a sterile, slightly beveled 27-gauge needle was used to inject 12.5 µl of 0.2% CtB in sterile saline. Bilateral injections were made at the fifth intercostal space (25 µl of 0.2% CtB total). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… After trephination in the occipital bone, pressure injections with retrograde tracers, 5 % Fluoro-Gold (FG; Fluorochrome, Denver, CO, USA) or 1% cholera toxin B subunit (CTB, List Biological Laboratories, INC.), were made at restricted regions in the cerebellar cortex using …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…Mice were sensitised intragastrically using a blunt needle with 20mg whey (DMV International) in 0.5ml PBS, with 10 mg cholera toxin (CT; Quadratech Diagnostics) used as an adjuvant (DMV International and List Biological Laboratories, Inc.), while sham mice received …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Chemicals and Reagents:

All toxins were purchased from List Biological Laboratories. …

FRET MAPKKide LF activity:

For drug testing in 96-well plates, the reaction volume was 250l per well, containing 20mM HEPES pH 7.2, 5M MAPKKide conjugated with DABYL and FITC (List Biological Laboratories, Inc), and …

Toxins Treatments:

Cells were seeded in a 96-well plate at a density of 10,000 cells/well 1 d before toxin treatment. Various concentrations of LF or FP59 combined with a fixed concentration of PA (500ng/mL) were added to the wells, and cells were incubated for 6h at 37C. Cell viability was measured by MTT assay. In other toxin treatment experiments, cells were pre-treated with various concentrations of drugs, and then treated with constant concentrations of 8.3g/ml of C. difficile toxin B, 500ng/ml of P. aeruginosa Exotoxin A, or 500ng/ml of Cholera toxin for 6hours. RAW264.7 survival was measured by MTT assay. …

• Product #155 – Toxin B from Clostridium difficile

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Mice and diabetes induction:

… To induce diabetes, male NSG RIP-DTR mice (812 weeks old) were injected intraperitoneally (ip) with 20 ng diphtheria toxin (List Biological Laboratories, Campbell, CA, USA) diluted in sterile phosphate-buffered saline (PBS). …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

CCR2⁺ monocyte depletion:

CCR2⁺ monocytes were depleted in CCR2-DTR mice and control CCR2-DTR negative littermates by i.p. administration of 250 ng of diphtheria toxin 2.5 days post CPS vaccination…Diphtheria Toxin was purchased from List Biological Laboratories (Campbell, CA), and reconstituted at 1 mg/ml in PBS. Aliquots were stored in 80C. Spleens were harvested on D5 post CPS vaccination, and tgd057-specific CD8⁺ T cells were analyzed.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Evidenciado a completa anestesia do animal, eles foram submetidos a uma injeo intra-ocular unilateral de 80 µl de uma soluo aquosa do traador neuronal antergrado, a CTb (CTb, List Biological L aboratories, Inc., Campbell, CA ) a 0,1%, contendo dimetilsulfxido a 10 % para aumentar a permeabilidade e consequentemente, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Cells were treated with dimethyl sulfoxide at a final concentration of 0.5% and used as a vehicle control. Purified LPS from Escherichia coli was purchased from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Diphtheria Toxin Injections:

Diphtheria toxin (DT) (List Biologicals) was diluted in PBS at a concentration of 10 g/ml. 1 g (100 l) of DT was injected intraperitoneally (i.p.) into Cx3cr1-Cre/+; Rosa-iDTR/+ mice (n = 6) every other day for 7 days.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… To induce AIA, mice were firstly immunized by subcutaneous injection of 100 µg methylated bovine serum albumin (mBSA, Sigma) in 100 µl complete Freunds adjuvant (CFA, Sigma) that contain ed 5 mg/ml heat – killed Mycobacterium tuberculosis strain H37Ra (Difco) into two sides on the hind flanks at day 0. On day 1, each mouse was intraperitoneally injected with 250 ng Pertussis toxin (List Bio Lab). At day 7 after immunization, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)