Citations

Reagents:

… LPS (Escherichia coli-derived, serotype O111:B4; List Biological Laboratories, Campbell, CA);…

Generation of ₂GPI-specific T Cell Hybridoma C3hB-1.5:

The C3hB-1.5 T cell hybridoma was generated from a C57BL/6 mouse that had received four biweekly intravenous immunizations of human ₂GPI (20 g) on Day 1 and LPS (10 g) on Day 2 as described previously…

• Product #201 – LPS from Escherichia coli O111:B4

Histology and immunohistochemistry:

For the CtB experiment, CtB-injected animals were perfused 7 d after CtB injections into the LH, and an antibody directed against CtB (1:100; List Biological Laboratories) was used to localize AcbSh neurons that project into the LH.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

… Lipopolysaccharide (LPS) was obtained from List Biological Laboratories (Campbell, CA). TNF-, IL-1, and IFN- were used separately, as a mixture (referred to as CytoCombo), or as a mixture of CytoCombo and LPS (CytoCombo + LPS)

Fractionation of endothelial cell-derived extracellular vesicles (E-EVs):

Ten-centimeter dishes coated with type I collagen (BD Biosciences, San Jose, CA) of confluent b.End5 cells were rinsed with PBS, and stimulated with medium containing CytoCombo + LPS, CytoCombo, or LPS alone to induce inflammation.

Scanning electron microscopy:

Endothelial b.End5 cells were seeded on cover slips and cultured until confluent. Confluent cells were then exposed to inflammatory stimuli (CytoCombo + LPS) for 10 seconds or 10 minutes. After stimulation, the cells were fixed with 2% formaldehyde…

Western blotting:

For E-EV marker analyses, E-EVs that were fractionated from the culture media of b.End5 cells after CytoCombo + LPS stimulation were used as sample.

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

DC Depletion with Diphtheria Toxin:

Mgl2-DTR and huLangerin-DTR mice were i.p. injected with 1 mg of diphtheria toxin (List Biological Laboratories) 2 days before infection,…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

…and LPS was from List Biological Laboratories (Campbell, CA).

LPS-induced apoptosis of DCs:

To induce apoptosis of DCs in vivo, RAG22/2 and RAG22/24-1BB2/2 mice were injected i.v. with 10 mg LPS and subsequently administered s.c. with 5 3 105 MC38 tumor cells. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

…On days 0, 3, and 7 each mouse was injected ip with 0.2 ng of purified Bordetella pertussis toxin (List Biologicals, Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… After the caudal NA was located by antidromic stimulation of the vagus (1015 mV, 0.2 ms), cholera toxin B subunit (CTB; List Biological Laboratories, Campbell, CA) was microinjected (1%, 20 nl) into the NA at 0.5 mm caudal to the obex. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials and Methods:

Animals – All experiments were performed according to international guidelines and approved by the local experimental animal committee of the University Medical Center Utrecht (DEC-ABC, Utrecht). On postnatal day 9 (P9), C57Bl/6 mice pups were anaesthetized with isoflurane (4% induction, 2% maintenance), the right common carotid artery was occluded by thermo-cauterization, xylocaine (100 mg/ml; AstraZeneca, Zoetermeer, The Netherlands) was applied and the incision was closed. After a minimal 1 h recovery, the pups were exposed to 10% O₂ in N₂ for 45 min and returned to their dams. Sham-control animals underwent anesthesia and incision only. LPS+HI or LPS+sham control animals received an intraperitoneal (i.p.) injection of LPS (List Biological Laboratories, Campbell, Calif., USA) at a dose of 0.5 mg/kg 14 h before surgery. …

Author has specified the following LPS product used:

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5 (Lot#4231A1)

Immunohistochemistry:

Tissue sections were incubated for 30 min at 37C in PBS, followed by 30 min in 0.2 m HCl and 1 mg/ml pepsin (Fisher Scientific), then permeabilized and blocked as described above. Sections were incubated for 3 nights at 4C in a mixture of primary antibodies against Cholera toxin subunit B (List Biological Catalog #703, RRID:AB_10013220; 1:5000) and…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Antibodies and ELISA assays:

Heavy-chain specific goat anti-human IgA-HRP was purchased from InvivoGen (San Diego, California, USA). ELISA assays for detection of anti-tetanus toxoid or anti-EEHV1A gL antibodies were carried out as described previously [13]. Briefly, ninety-six well polystyrene plates (Immulon 4 HBX, Thermo Electron Corp., Milford, MA) were coated with either 1 ug of purified tetanus toxoid (List Biological Laboratories,US) at a 20 ug/mL concentration…

• Product #191 – Tetanus Toxoid from Clostridium tetani