Bacterial Toxin Research Citations

…In six rats, BDA was injected into the MPB (n = 3) or MPBE (n = 3) as described above, and 0.5% cholera toxin B subunit (CTb; List Biological Laboratories, Campbell, CA, USA) in phosphate buffer was injected unilaterally into the CPu by iontophoresis (positive, 4 A, 2 Hz, 15 …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE Model:

Our EAE model was designed with reagent concentrations that produce a mild-to-moderate form of EAE, always yielding clinical scores of motor dysfunction below 3 and 4 over the 4 week testing period [34]. All animals in the current study exhibited neither paresis of both hindlimbs nor paralysis of one hindlimb, thus allowing nociceptive paw reflex testing. … MOG was bilaterally injected (150 µg/100 µl s.c.) at the flanks under light isoflurane anesthesia on Days 0 and 6. Pertussis toxin (List Biological Laboratories, Campbell, CA) was injected (200 ng/200 µl, i.p.) on days 0 and 2 to facilitate opening of the blood brain barrier to T cells by histamine-induced vascular leakage and/or the priming of autoreactive T cells [16]. Control mice received CFA and pertussis toxin, but not MOG. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Blocking neutrophil migration from skin:

Microbial inflammation was induced as above, and, 4h later, ear pinnae of photoconvertible mice were injected using a 29-gauge needle with 5 µg of anti-CD11b, (clone M1/70), …or 500ng of pertussis toxin (List Biological Laboratories) or PBS control, immediately before photoconversion.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and the EAE model:

C57BL/6 mice were bred … Six to ten-week-old female mice were utilized for experiments. … Mice were injected i.p. with 200 ng of pertussis toxin (List Biological Laboratories) immediately following MOG₃₅₅₅ injection (day 0) and again 2 days post immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Pertussis toxin (List Biological Laboratories, Inc.) at a dose of 200 µg per mouse in PBS was administered intraveously on the day of immunization and once more 48h later. The disease score was evaluated as previously described (ref. 49): 0, no clinical signs;

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Proteins and peptides:

Immobilized PA83 (LIST Biological Laboratories, Inc.) was used in the ELISA to establish titers of PA-reactive Ab in the clinical samples. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Antigen challenge to skin:

… For OVA + CT immunization, mice were immunized epicutaneously as previously described8. Briefly, we used scotch tape (3M) to gently remove the cornified layer of ear skin, and then treated the skin with acetone and cholera toxin adjuvant (List Biological Labs) before administrating chicken ovalbumin + cholera toxin.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

For mCherry expressing Salmonella infection, mice were infected intranasally with 10 9 CFU per mouse. Where specified, 1µg pertussis toxin (List Biological Laboratories, Campbell, CA) per mouse was supplemented with Salmonella.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

LPS from E. coli O55:B5 was purchased from List Biologicals (Campbell, CA, USA). 

Endotoxemia:

Mice were injected via tail vein with 40 g of LPS (2 mg/kg) or 4 mg of purified mouse hemoglobin or both in a volume of 200 l,…

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Depletion of pDCs:

Recipient animals were pDC depleted with DT (100 ng/mouse, intraperitoneally [IP], 3 times per week; List Biologicals, Campbell, CA). OVA-speci fi c Treg stain with MR9-4 ant ibody (eBiosc ience) and transferred OT-II cells are identi fi ed as CTV 1.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae