Citations

Induction of EAE:

… Mice were concurrently injected twice with 200 ng of pertussis toxin (List Laboratories, Campbell, CA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

PdpnTG mice (552), WT mice, Pdpnfl/flCd4-Cre mice, Pdpnfl/fl mice, and Foxp3.GFP KI mice were injected s.c. with 100 µg MOG3555 peptide emulsified…Mice also received 100 ng pertussis toxin (List Biological Laboratories Inc.) i.v. on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents and antibodies:

… Un-nicked Diphtheria Toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA) and was used to deplete Foxp3⁺ Tregs from DEREG mice [49] by administering 1g i.p. DT per two days from day -2 to day six p.i. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

CCR2⁺ cell depletion strategy:

For depletions of CCR2⁺ cells, CCR2-DTR mice and control littermates received 250 ng of diphtheria toxin i.p. one day prior to infection. Diphtheria toxin was purchased from List Biological Laboratories (Campbell, CA) and reconstituted at 1 mg/ml in PBS. Aliquots were stored at 80C. The specificity and efficiency of depletion in the lung was confirmed by flow cytometry.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Prior to the start of surgery, animals were injected in the left hind quarter with ketoprofen (KetofenTM, Fort Dodge Animal Health, Fort Dodge, IA; 5 mg/kg S.C.). Animal were anesthetized with isoflurane (5% induction and 2% for maintenance, Henry Schein, Melville, NY). In order to label spinally projecting neurons in RVLM, each group of rats received an injection of cholera toxin B subunit (CTB, 1%, List Biological Laboratories, Inc., Campbell, CA), a retrograde tracer, into the T9-T10 region of the spinal cord as in previous studies (Quote Nicks and the NR1 paper). Briefly, rats were put in a stereotaxic head holder and the T9-T10 region of the thoracic spinal cord was exposed. CTB (3 X 30 nl) was microinjected bilaterally into the T9-T10 of the spinal cord. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunology:

Anti-CT and -LT IgG and IgA antibodies were measured by enzyme-linked immunosorbent assay (ELISA) (18).  The critical reagents used in these assays were as follows: cholera toxin B subunit (catalog no. 103B, lot no. 10327A, 1.0 mg; List Biological Laboratories, Inc., Campbell, CA), which was resuspended in 0.2 ml deionized (DI) water for a final concentration of 5.0 mg/ml, and stored at 2 to 8C; …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Unilateral pressure injections of 10% biotinylated dextran amine (BDA; Molecular Probes; in distilled water) for adults or 1% cholera toxin subunit B (CTB; List Labs) for P10 animals were made into the left hemisphere, and a survival period of 57 days was allowed for dye …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Infection, Induction and Scoring of EAE:

Female 6- to 8-week-old BALB/c mice were used throughout this study. New-born mice 6 to 12 h postpartum were inoculated i. p. with 200 PFU of wild-type MCMV (MW97. 01 strain) or 200 µl of phosphate buffered saline (PBS) as a control. EAE was induced via subcutaneous administration of 200 L of a suspension at 2 sites above the hind flanks. … Each mouse was immediately injected intraperitoneally and 48 hours later with 300 ng of pertussis toxin (List Biological Laboratories, Campbell, USA) in 100 µL of 0.9% NaCl. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Assessment of EAE:

… Mice also received 200 ng of pertussis toxin (List Biological Laboratories) i.p. in 0.2 ml of PBS on the same day and 2 days later. Clinical signs of EAE were assessed daily with a 06 scoring system (0, no signs; 1, tail limpness; 2, impaired righting re- flex; 3, hind limb weakness; 4, hind limb paralysis; 5, hind limb paralysis with fore limb weakness; 6, death). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Double Tracing of CST and Propriospinal Neurons:

…Retrograde tracing of LPSNs was carried out at the same time by injecting 1 µl of cholera toxin subunit B (CTB; 1%; List Biological, Campbell, CA, USA) into each side of the lumbar spinal cord …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt