Bacterial Toxin Research Citations

Induction of chronic relapsing EAE and neurological assessment:

Female C57BL/6 mice (8-12 weeks of age) were purchased from Charles River Laboratories. Mice were injected subcutaneously (s.c.) in the back with 200µL of recombinant MOG_35-55 (200µg, Biomatik Corporation, Cambridge, ON, Canada), which was emulsified in incomplete Freund’s adjuvant (Sigma, Oakville, ON, Canada) and supplemented with 4 mg/mL Mycobacterium tuberculosis (strain H37Ra, BD Biosciences, Missisauga, ON, Canada) in four different sites (50µL per site). Two hundred nanograms of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA) was injected intraperitoneally (i.p.) on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mycobacterium tuberculosis (H37Ra strain; BD Diagnostics). On the day of immunization and 48 h later, the mice were also injected iv with pertussis toxin (200 ng/mouse; List Biological Laboratories) in PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction in mice:

…A total volume of 100 µl was injected subcutaneously (s.c.) on each side of the tail base. The mice were also given 200ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitonally (i.p.). Another 200ng of pertussis toxin was given 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

On day 0 and 2 after immunization, 200 ng pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA) was given intravenously. Animals were scored every 23 day …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Priming of antigen-specific T cells

… Control mice were injected s.c. bilaterally with an emulsion of PBS and CFA. 100 ng PTx (List Biological Laboratories, Inc., Campbell, CA, USA) was administered i.p. on days 0, 1 and 2 to induce EAE.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…To induce EAE, the peptide of myelin proteolipid protein (PLP 139151 ) was subcutaneously injected into female SJL/J mice (Jackson Laboratory, 6-8 weeks old), and pertussis toxin (List Biological Laboratories, Inc.) 200 ng was injected intravenously on the day of …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Short-term homing:

… CMRA-labelled control cells were incubated with 300 ng/ml pertussis toxin (List Biological Laboratories, Campbell, CA) for 2h. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

…LF, EF, PA83, and PA63 were obtained from List Biological Laboratories (Campbell, CA). …

Animal care and study protocol:

…Total LF and LTx analysis was determined in two animals, 16N and 89N, over the course of infection. …

Antibody specificity:

Specificity of anti-PA and anti-LF MBs was demonstrated for LTx, LF, and PA. PA63/LF were mixed at a w/w ratio of 10:1 to form LTx complex. PA83 and LF were mixed as a non-complexed control. Mixtures were incubated at room temperature (RT) for 30 min. LF, PA63, PA83, LF/PA63, and LF/PA83 at 0.5 g LF and 5 g PA83 or PA63 were immunoprecipitated with anti-LF and anti-PA MBs, …

Lethal toxin standard preparation and characterization:

LTx was formed by mixing PA63 and LF at w/w ratios of 0:1 to 60:1 PA63/LF, …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

STEC cultures and Shiga toxins (Stx): Purified Stx1 and Stx2a were purchased from List Biological Laboratories (Campbell, CA, USA). …

• Product #161 – Shiga Toxin 1 from Escherichia coli
• Product #162 – Shiga Toxin 2 from Escherichia coli

… Purified CT (Cat. No. 100B) and diphtheria toxin (Cat. No. 150) were purchased from List Biological Laboratories (Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae