Bacterial Toxin Research Citations

Adjuvant activity of LP1569:

Mice were immunized intraperitoneally twice (0 and 4 or 5 weeks)…and pertactin, PT, and recombinant PT were obtained from List Biological Laboratories (Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

• Product #184 – Pertussis Toxin Mutant
• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

MOG35-55 peptide and SRBC immunizations:

Mice were immunized with 100-150 µg of MOG35-55 peptide (Genemed Synthesis)subcutaneously (s.c.) in an emulsion of complete Freuds Adjuvant (CFA) containing 1mg/mL of heat killed H37RA strain of Mycobacterium tuberculosis (Sigma-Aldrich) in the hind leg region. Pertussis toxin (List Biological Laboratories, Inc) in PBS was injected intraperitoneally (i.p.) at a dose of 100-250 µg on the day of immunization and again 2 days after. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

To induce EAE, J18^/, CD1d^/, IL-4^/ and WT C57BL6 mice were injected subcutaneously with 200 µg myelin oligodendrocyte glycoprotein (MOG)3555 (amino acid sequence, MEVGWYRSPFSRVVHLYRNGK; Anaspec, Fremont, CA, USA) emulsified in Freunds adjuvant (Difco, Detroit, MI, USA) supplemented with attenuated Mycobacterium tuberculosis H37RA (Difco) to 4mg ml¹. On days 0 and 2 after immunization, the mice were injected intraperitoneally (i.p.) with 200ng pertussis toxin (List Biological Laboratories; Campbell, CA, USA) in 100 l PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 200 ng pertussis toxin (List Biological Laboratories, …) in 500 µl sterile PBS (PAA, Pasching, Austria) was administered on days 0 and 2 post immunization. Clinical signs were evaluated …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.6. Induction of experimental autoimmune encephalomyelitis (EAE) and FTY720 treatment:

68 weeks old female C57BL/6 WT and Cbl-b^/ mice were immunized s.c. in the back with 165 µg of MOG₃₅₅₅ emulsified with CFA containing 300 µg of H37RA mycobacteria (Difco BD). 150 ng of Pertussis toxin (PTX) (List Biological Laboratories, Campbell, CA) was administered i.p. on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

For active EAE, mice were immunized s.c. on the back with 200 mg MOG3555 (MEVGWYRSPFSRVVHLYRNGK) emulsied in CFA…Two hundred nanograms of pertussis toxin (List Biological Lab,…) was given i.p. on days 0 and 2 postimmunization (p.i.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis:

Experimental autoimmune encephalomyelitis (EAE) was induced in 57-week-old C57BL/6 females by subcutaneous immunization with 100 µL of an emulsion of 200 µg MOG₃₅₅₅ peptidE…An intraperitoneal injection of 200 ng pertussis toxin (List Biologicals) was administered on the day of priming and 2 days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin (PTx) was from List Biological Labs (Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction and Clinical Evaluation:

…The mice were also injected intraperitoneally with 20 µg/kg of pertussis toxin (List Biological Laboratories) immediately and 2 days after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

EAE was induced in 8- to 12-week-old mice by immunization with 100 µg rhMOG in complete Freund adjuvant containing 200 µg Mycobacterium tuberculosis H37RA (DIFCO Laboratories, Detroit, MI) on day 0. Mice intraperitoneally (i.p.) received either 100ng (Fig 1) or 200ng (all other experiments) Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)