Bacterial Toxin Research Citations

Cell isolation and propagation:

Adjacent normal lung tissue from a surgical specimen of lung tumor was obtained with the written informed consent of the patient. …1 nM cholera toxin (List Biological Labs), …All cells were maintained at 37°C in a humidified incubator, with 5% CO2. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

siRNA, antibodies and reagents:

Pertussis toxin (PTX)…was purchased from List Biological Laboratories.

Active systemic anaphylaxis:

Active systemic anaphylaxis model was achieved as previously described²⁰: five mice were immunized with i.p. injection of 1mg BSA and 300ng pertussis toxin as adjuvant in normal saline.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Culture of porcine urothelial cells:

Porcine urothelial cells (PUC) were isolated either from bladder biopsies or ureter specimens of minipigs under sterile conditions. … Isolated cells were washed in keratinocyte serum-free medium (KSFM; Invitrogen), supplemented with 50 µg/ml bovine pituitary extract, 5 ng/ ml human recombinant epidermal growth factor (both Invitrogen), 30 ng/ml cholera toxin (List Biological Laboratories), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture:

For primary adult microglia culture CNS mononuclear cells were isolated from brain and spinal cord of 46 week old mice under sterile conditions and cultured in VLE-DMEM (Biochrom) with 10% FCS, 50 M -ME and 15% of M-CSF containing supernatant from L929 cells. The protocol was adapted from Ponomarev [21]. Cells were stimulated on d14 with 50 g/ml poly (I:C), 6 g/ml CpG2216 (TIB MOLBIOL), 100 ng/ml Lipopolysaccharide (LPS) from Salmonella minnesota R595 (List Biological Laboratories, Inc.), …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Mice:

For Cre activation, tamoxifen…was given via gavage to neonatal mice (0.075mgg¹ for Lgr5EGFP-CreERT2 and 0.75mgg¹ Plp1-CreERT strains) and dams (0.225mgg¹, i.p.). Diphtheria toxin (6.25ngg¹ i.p., List Biological Laboratories)…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

To identify the retinofugal projection, 4 days before sacrifice, 2.5 m lof the orthogradely transported tracer CTB was intravitreally injected (1%, diluted in distilled water; List Biological Laboratories, Campbell, CA) following previously described protocols that are standard in our laboratory …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Female BALB/c mice (n = 10) were sensitized to EW by oral gavage (5 mg of protein plus 10 µg of cholera toxin List Biologicals, Campbell, CA per mouse), as described by Jimnez-Saiz, Rupa, and Mine (2011). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Intraperitoneal (ip) injections of pertussis toxin (200 ng) in phosphate buffered saline (List Biological Laboratories, Campbell, CA) were given on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Culture: Human Oral Mucosal Epithelial Cell Sheets:

…1 nM cholera toxin (List Biological Laboratories, Inc., Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…Lipopolysaccaride (LPS) from Salmonella typhimurium was from List Biological Laboratories (Campbell, CA). …

• Product #225 – LPS from Salmonella typhimurium