Bacterial Toxin Research Citations

Reagents and antibodies:

Cholera toxin (Product #100)…were from List Biological (Campbell, CA, USA)…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials and methods

Cell culture:

…100 ng/mL cholera toxin (List Biological Laboratories),…

Three-dimensional morphogenesis assay:

Forty microliters of Matrigel (growth factor reduced, BD) was added to each well of 48-well plates and then the plates were incubated at 37­°C to allow solidification of the Matrigel. MCF10A cells were trypsinized and resuspended in assay medium (DMEM/F12 supplemented with…100 ng/mL cholera toxin, …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

LPS from Escherichia coli K12 D31m4 (Re) was obtained from List Biological Laboratories (Campbell, California, USA).

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

… Then, cells were harvested and seeded into six-well plates. LPS (List Biological Laboratories, Campbell, CA) was usually given at a dose of 1 g/mL. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

RNAseq analysis to monitor the effect of SF3a1 inhibition:

The RAW264.7 mouse macrophage cell line was transfected with either SF3a1 siRNA or control non-targeting siRNA (Dharmacon) using the 96-well shuttle transfection system (Amaxa) as described previously [26]. Twenty-four hours later, cells were exposed to either 20 ng/ml LPS (List Biological labs), or not as a control.

Author has specified that the following LPS product was used:

• Product #201 – LPS from Escherichia coli O111:B4

… Lipopolysaccharide (LPS) from Escherichia coli 0111:B4 was obtained from LIST Biological Laboratories (Campbell, CA, USA). …

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

…Each mouse was immunized with a subcutaneous injection of 100 µl MOG 3555 /CFA emulsion (containing 200 µg MOG 3555 ) and an intraperitoneally injection with 200 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) on the same day and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Model:

…On day 0 of the study, the SJL/J mice were induced with EAE through four 50µL injections of an emulsion containing Incomplete Freund’s Adjuvant Oil…Each animal was also given a 100µL i.p. injection of 200 ng of pertussis toxin (List Biological Laboratories, Inc., Campbell, California) on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAE:

Mice were injected subcutaneously (s.c.) in both flanks with 100 µg myelin oligodendrocyte glycoprotein (MOG)_35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) dissolved in PBS emulsified in an equal volume of CFA (Difco) supplemented with 5mg/ ml¹Mycobacterium tuberculosis H37Ra. Pertussis toxin (PT; List Biological Laboratories) was injected i.p. (150ng) on the day of immunization and 48h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

EAE was induced by subcutaneous immunization in the flanks with 100µg of MOG_3555.…In addition, mice received intraperitoneally 200ng of pertussis toxin (List Biological Laboratories) on days 0 and 2 post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)