Bacterial Toxin Research Citations

Induction and evaluation of EAE:

To induce relapse EAE, SJL.J mice were immunized with 150µg of PLP₁₃₉₁₅₁ in CFA containing 5mg/ml¹ heat-killed Mycobacterium tuberculosis. For both types of induction, on the day of immunization and 48h later, the mice were also injected i.v. with Pertussis toxin (200ng per mouse; List Biological Laboratories, Campbell, CA) in PBS. Mice were examined daily for EAE disease symptoms, which were scored using a…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde labelling:

2.1 Tracer injection

The injections of CTB (low salt, List Biological Laboratories, CA, USA) were performed 50-69 days after the undercut surgery. The procedures of injection and immunohistochemical reactions were similar to those recently described (Ahmed et al., 2012). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

…LPS from E. coli O111:B4 for in vitro experiment (List Biological Laboratories).

Immunoblotting:

Macrophages were treated with LPS, and cell lysates were analyzed to detect the phosphorylation of kinases by immunoblotting.

Flow cytometry:

Peritoneal macrophages were treated with LPS to induce the expression of 4-1BBL at the cell surface. …

• Product #201 – LPS from Escherichia coli O111:B4

EAE Induction and Clinical Evaluation:

In addition, the mice received 200µg of pertussis toxin (List Biological Laboratories) intravenously on the day of, and 2 days after, peptide immunization. Individual animals were graded according to clinical severity as follows: grade 0, no abnormality; grade 1, limp tail; grade 2, limp tail and hind limb weakness; grade 3, partial hind limb paralysis; grade 4, complete hind limb paralysis; as previously described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tracer injection:

Five days before being killed, the animals (rats) were injected into the sciatic nerve (11 bilaterally, 30 unilaterally) with a 1% wt per vol solution of the transganglionic tracer CTB (List Biological Laboratories, Campbell, CA) using a 36-ga NanoFil syringe. The sciatic nerve was exposed as described above and 1 µl was injected into each the tibial and common peroneal branches bilaterally (4 µl total).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE induction and antibody treatment:

Active EAE was induced by subcutaneous immunization of mice with an emulsion of 10 µg of MOG₃₅₅₅ peptide in CFA supplemented with 40mg of M. tuberculosis extract H37 Ra (Difco). In addition, the animals received 200ng of pertussis toxin (List Biological Laboratories) i.p. on day 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… in Complete Freund’s Adjuvant (Sigma-Aldrich). At the same time mice were also administered 250 ng of pertussis toxin (List Biological Laboratories) ip and, when indicated, again on d2. Clinical disease was monitored daily …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

BoNT-B:

The light chain of botulinum neurotoxin type B (50 kDa, List Biological Laboratories, Campbell, CA) was used to cleave recombinant Sb2.13

• Product #620A – Botulinum Neurotoxin Type B Light Chain, Recombinant

… CTB-labeled sensory axons can be identified by immunostaining with a goat anti-CTB IgG antibody (1:4,000, List Biological Laboratories, Campbell, CA, USA. # 703). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Treatment of EAE by DiD-Labeled MSCs:

MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK, Peptron, Korea) was used to induce chronic EAE in C57BL/6 mice [18]. Briefly, 200 µg of MOG₃₅₅₅ diluted in 200 µL PBS and emulsified with 200 µL incomplete Freunds adjuvant (Gibco, Germany) and 1mg/mL heat inactivated mycobacterium tuberculosis (Difco, USA) in PBS was injected subcutaneously. After MOG₃₅₅₅ administration, 200ng pertussis toxin (List Biological Laboratory, USA) was injected intraperitoneally on the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)