Bacterial Toxin Research Citations

Experimental autoimmune encephalomyelitis (EAE) induction:

SJL/J mice were injected subcutaneously in the flanks with 200 ml of 1 mM PLP₁₃₉₁₅₁ peptide. The emulsion solution was reconstituted complete Freunds adjuvant (CFA), composed of Freunds incomplete adjuvant (Pierce Biotechnology, Rockford, IL) containing Mycobacterium tuberculosis H37 Ra (2 mg/ml) (Difco Laboratories, Detroit, MI), and PLP_{139 151}. Mice were intravenously injected with 0.2 mg of Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA), in a 100 ml final volume, on days 0 and 2 following sensitization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

BrdU and PTX treatment:

… Mice were treated with 1 mg/ml BrdU (BD) i.v. and administered 1 mg/ml BrdU (Sigma-Aldrich) in drinking water 24 h before analysis. 1 µg PTX (List Bio Labs) was injected i.v. 24 h before analysis. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… On day 0, mice were immunized s.c. with 100 µg MOG35-55 peptide (BEX) emulsified in CFA (Sigma-Aldrich) at the base of the tail. The mice received i.v. 100 ng pertussis toxin (List Laboratories) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin B-subunits, List Biological Laboratories, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Retrograde tracing experiment:

Rats were anesthetized with a mixture of ketamine …Animals were placed in a Stoelting stereotaxic frame and a hand drill was used to expose the brain surface above the target sites. Iontophoretic injections of 0.5% CTb (List Biologicals, Campbell, CA, USA), which was dissolved in 0.01 M phosphate buffer (PB; pH 7.4), were done by applying a positive current of 1.02.5 A (200 ms pulses at 2 Hz for 15 min) through a chlorinated silver wire placed in a glass pipette (approximately 7µm diameter tip). The coordinates used for injecting CTb were as follows: aPVT at 1.5 mm posterior, 1.0 mm lateral, 5.0 mm ventral, 10 angle toward the middle; and pPVT at 3.0 mm posterior, 0.7 mm lateral, 5.0 mm ventral, 10 angle toward the middle (all coordinates are relative to bregma and the dural surface of the …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

MOG-induced EAE: active induction of EAE

… C57BL/6J and Gas6^/ mice were immunized with MOG_35-55 peptide. MOG_35-…Mice were anesthetized with isoflurane and 100 µl of emulsion was injected subcutaneously on each flank (200 µl total/mouse) on day 0. In addition, 200 µl of pertussis toxin (Ptx, 2.5 µg/ml; List Biological Laboratories) was injected intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

Anthrax lethal factor (batch 1692A1B), and protective antigen (batch 17117A1B) from List Biological Laboratories.

Discussion:

In mouse macrophages of sensitive strains, LT triggers the formation of the NLRP1b inflammasome, culminating in pyroptosis and in the secretion of mature IL-1 and active caspase-1…

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

List Labs Product #169A (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Lyophilized) has been discontinued; however, Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence; Liquid) is available for purchase.

• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

… In addition, there are several versions of FRET based peptides available in market (List Biological Laboratories, INC. USA), and one of them has been claimed to exhibit a LOD as low as 140 pg/mL after 5 h digest or 8 pg/mL after 24 h digest. …

Author did not specify which List Labs FRET peptides product was utilized in their research.  List Labs provides the following FRET peptides, Native Substrates and Receptors:  https://listlabs.com/product-information/fret-peptides-native-substrates-receptors/

EAE induction:

… Mice were immunized via subcutaneous (s.c.) injection with 100 µg MOG₃₅₅₅ emulsified in CFA on day 0. Mice also received 250 ng Pertussis toxin (List Biological Labs) i.v. via tail vein injection at the time of MOG₃₅₅₅/CFA immunization and again 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

To measure tetanus IgG titres and to determine the IgG subclasses, we randomly selected plasma samples from eight paired HbAA and HbAS pairs of children in each age group (3-5, 6-8, and 9-11 years) and tested them at A dilution of 1:500 (n = 48). Tetanus toxoid was purchased from the List biological Laboratories, Inc. (Campbell, CA).