Bacterial Toxin Research Citations

Anti-toxin A/B IgG measurement:

Levels of IgG specific to C. difficile toxin A and toxin B were measured in serum from infected mice. 96-well EIA plates (Corning Incorporated, Tewksbury, MA) were coated with 100l of either purified C. difficile toxin A or toxin B (List Laboratories, Campbell, CA) at 5g/mL in …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

… Two weeks after weaning, mice were sensitized intragastrically (ig) with 20 mg whey (DMV International, Veghel, The Netherlands) in 0.5 mL PBS containing 10 µg cholera toxin (CT, List Biological Laboratories, Campbell, CA, USA) as an adjuvant. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Maternal protocol

To establish maternal peanut allergy, female C3H/HeJ mice, on a peanut free diet, were orally sensitized with peanut as previously described(15;16) with slight modification. Briefly, 6 week-old female mice were sensitized with peanut (10 mg PN) and cholera toxin (20 μg CT) intragastrically (i.g.) weekly for 5 weeks (wks) and challenged at wk 6 (200 mg PN/mouse). Our sensitization protocol differed from our previous standard protocol in that mice did not receive an additional boost (50 mg PN+10 mg CT). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Protein stability:

… For in vitro studies: Stx1 (5 µg/ml) (BEI resources, Cat.#NR-857), Stx2a (BEI resources, Cat.#NR-4478) (5 µg/ml), cholera toxin (5 µg/ml) (List Biological, Cat.#101B) or supernatants were concentrated to a final volume of less …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Toxina Colrica 10mg/ml List Biological Laboraries …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Sensitization of mice:

… For sensitization, Il4raF709 and Il4raF709/IgE-deficient (IgE / ) mice were treated intragastrically with sterile PBS or 250 µg of ovalbumin (OVA; Sigma-Aldrich, St Louis, Mo) plus 20 µg of cholera toxin (List Biological Laboratories, Campbell, Calif) once a week for 3 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry:

After sectioning, free-floating brainstem sections were washed for 30min in a 1:1 pretreatment solution of Triton + PBS (TPBS; 1:200) and hydrogen peroxide. Between each incubation step, sections were rinsed 35 minutes in PBS. Sections were blocked for 1 hour in 10% normal donkey serum (NDS) in PBS. Sections were removed from blocking solution and placed directly into primary antibody for incubation at room temperature (Goat -CTB; List Biologicals, Campbell, CA, USA; 1:40,000 in PBS). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

… phenol – extracted LPS ( Escherichia coli O111:B4) that is free of lipoprotein contamination (List Biological Laboratories); …

• Product #421 –  ULTRA PURE LPS from Escherichia coli O111:B4

Western Blotting:

In Vitro Studies:  Naive wild-type and/or TLR2-/- Ms seeded in 6-well plates (2 x 106 per well) were stimulated with either viable Bb (MOI = 10) or Salmonella typhimurium LPS (200 ng/ml) (List Biological Laboratories). At indicated times post-stimulation, cells were collected and lysed for total protein extracts. …

• Product #225 – LPS from Salmonella typhimurium

Materials and Reagents:

… Lipopolysaccaride (LPS) from Salmonella typhimurium was from List Biological Laboratories (Campbell, CA). …

Culture of Extraplacental Choriodecidual Membranes:

… LTA and LPS treatments were initially dissolved in water and diluted in DMEM/1% FBS. Following acclimation, …

• Product #225 – LPS from Salmonella typhimurium