Citations

Animals:

Two male macaque monkeys, one Macaca fascicularis (MF), aged 20, and one Macaca mulatta (MM1), aged 5, were used for the tracer study. Two male Macaca mulatta, one aged 8 (MM2) and one aged 2.5 years (MM3) were used for the detailed retina analyses. The animals were kept in the primate animal facility of the University of Alabama at Birmingham in 12:12 light:dark conditions. For the tracing study, MM1 and MF received a unilateral injection of 100 l of 1% cholera toxin B (CtB) (List Biological) in sterile water into the vitreous body using a 1cc tuberculin syringe under isoflurane anaesthesia. …

Antibody Characterization:

…A commercially available anti-CtB antibody (code no. 703, Lot#7032E, List Biologicals, Champbell, CA, USA, diluted 1:1000) raised in goat was used for tracer visualization. According to the manufacturer the antigen was a B subunit pentamer of cholera toxin (choleragenoid). Omission of the primary antibody abolished all staining (not shown). …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… LPS (E.-coli-derived, serotype O111:B4) was from List Biological Laboratories (Campbell, CA); …

• Product #201 – LPS from Escherichia coli O111:B4

EAE model induction and treatment:

EAE was induced as follows: mice (age 6 to 8 wk) were immunized subcutaneously (s.c.) with a mixture of myelin oligodendrocyte glycoprotein…Pertussis toxin (200 ng/mouse, List Biological Laboratories, 181) in PBS was administered i.v. on day 0 and day 2.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

Active optimal or sub-optimal EAE was induced with sub-cutaneous (s.c.) injection of 200 or 160 µg MOG3555 peptide (Peptides International) respectively in 150 µl of Complete Freunds Adjuvant (Sigma-Aldrich). On day 0 and 2 postimmunization, intraperitoneal (i.p.) injection of 400 or 300 ng of Bordetella pertussis toxin (PT; List Biological Laboratories) was respectively performed for optimal or sub-optimal EAE induction. EAE severity was scored daily using the standard scale. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE) mouse model:

… The mice in the EAE group were immunized with 50 µg MOG_35-55 peptide emulsified (1:1) in incomplete Freunds adjuvant (IFA) and desiccated Mycobacterium tuberculosis. Pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA) was injected intravenously on the day of MOG_35-55 immunization and two days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAE:

…On the day of immunization and 48 h later, the mice were also injected i.v. with pertussis toxin (200 ng/ mouse; List Biological Laboratories, Campbell, CA) in PBS. Mice were examined daily for EAE disease symptoms, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Each animal received bilateral injections of cholera toxin B subunit (CTB, choleragenoid, List Biological Laboratories, Campbell, USA) in either the entopallium or field L2. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

In vivo extracellular injections:

Injections of 310 nL of a 1% solution of cholera toxin subunit b (CTb) (List Labs, Campbell, CA) were performed in 10 pigeons. The targets of these injections were the eye chamber (two cases), the GLv (five cases), and the TeO (three cases). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… The tip of a glass canula sealed to a Hamilton syringe was inserted through the incision and 3.5 µl of a 1% cholera toxin B subunit solution (CTB; List Biological Laboratories) were slowly injected over a 3 min time period. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of EAM and DCMi:

On day 0, mice also received 500 ng pertussis toxin i.p. (List Biologicals).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)