Bacterial Toxin Research Citations

EAE induction and scoring:

EAE was induced by s.c. immunization with 200µg MOG_35-55 (MEVGWYRSPFSRVVHLYRNGK) emulsified in 500µg CFA (at 10mg/mL M. tuberculosis H37Ra, Difco) and i.p. injection of 200ng pertussis toxin (List Biological Laboratories, Inc.). Spleen and draining lymph node cells at 13 d post-immunization were taken and stained for flow cytometry analysis. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and Therapeutic Study:

…The PLP-induced model of EAE was used to mimic relapsing-remitting multiple sclerosis (29). Mice were immunized subcutaneously with 200 µg of PLP₁₃₉₁₅₁ in a 0.2 mL emulsion composed of equal volumes of phosphate-buffered saline (PBS) and CFA containing killed Mycobacterium tuberculosis strain H37RA… In addition, 100 ng of pertussis toxin (List Biological Laboratories Inc., Campbell, CA) was injected (100 µL) on two occasions, intraperitoneally on the day of immunization (day 0) and 2 days post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and Therapeutic Study:

Five- to 7-week-old SJL/J female mice were immunized subcutaneously (s.c). with 200 mg of PLP139151 in a 0.2 ml emulsion composed of equal volumes of phosphate-buffered saline (PBS) and complete Freunds adjuvant (CFA) containing killed Mycobacterium tuberculosis strain H37RA (final concentration of 4 mg/ml; Difco). …In addition, 200 ng/100 µL of pertussis toxin (List Biological Laboratories Inc.) was injected intraperitoneally (i.p.) on the day of immunization (day 0) and 2 days post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

Experimental autoimmune encephalomyelitis was induced in mice by active immunization with MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK), of murine origin… These mice were also injected i.p. with 500 ng pertussis toxin ([PTX] List Laboratories, Campbell, CA), in PBS, pH 7.4, on D0 and D2 after the first immunization (referred to as the MOG-CFA/PTX group).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction in mice:

… on day 0. 6 and 48 h later, the mice were administered i.v. with 500 ng of purified Bordetella Pertussis toxin (List Biological Laboratories, Campbell, CA). Disease severity was monitored according to the following scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis:

… Mice were immunized s.c. with MOG peptide 3555 (300 µg/mouse) and Mycobacterium tuberculosis (500 µg/mouse) emulsified in complete Freund’s adjuvant, in a volume of 0.1 ml/mouse over the right-draining inguinal and axillary lymph nodes…Pertussis toxin (500 ng/mouse; List Biological Laboratories, Campbell, CA) was injected intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Acute Optic Neuritis of EAE:

…For EAE-immunized mice, the adjuvant pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA) was injected intravenously on the day of immunization and two days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Antigen-induced arthritis:

… Simultaneously, 1 µg of pertussis toxin (List Biological Laboratories Inc, Campbell, CA, USA) was injected intraperitoneally as an additional adjuvant. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Corneal afferent tract tracing and capsaicin application techniques for immunocytochemistry studies:

2.3.1. Cholera toxin B subunit

(CTb; 1% solution in 0.1 M phosphate buffer (PB); List Biological Laboratories, Inc., Campbell, CA) was used as an anterograde tracer to identify central projections of corneal afferents into the ventrolateral dorsal horn of trigeminal nucleus caudalis (Vc) as previously described …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Animal Surgery and EEG/EMG Implantation:

… Injections of… or CTB, List Biological, Campbell, CA) were administered directly into the brain using a fine glass pipette (1 mm glass stock, tapering slowly to a 10-20 µm tip) connected to an air compression system. A series of 20-40psi puffs of air were used to deliver the compounds into and with the following coordinates and volumes: …, 33nL 1% CTB; …16.5nL 1% CTB…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt