Citations

… and PA was obtained from List Biological Laboratories (Campbell, CA, USA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Allergic Sensitization and Anaphylaxis:

Mast cells (mice) were depleted by i.p. injection of diphtheria toxin (List Biological Laboratories) in saline over 3 days: 100 ng day 1, 500 ng day 2, and 500 ng day 3.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Reagents:

For the bilayer lipid measurements, PA 63 was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA).

Channel Reconstitution into Planar Lipid Bilayers:

…Single channels were formed by adding 0.5 to 1 L of 20g mL 1 solution of PA63, or 0.2 to 0.5 L of 48 ng mL 1 solution of C2IIa to the 1.5 mL aqueous phase on the cis-half of the bilayer chamber. For multichannel experiments, we applied 12 L of 0.2 mg mL1 stock PA63 or 12 L of 48g mL 1 stock C2IIa to the cis-side of the membrane. Under this protocol, PA63 and C2IIa channel insertions were always
directional as judged by channel conductance asymmetry in the applied transmembrane voltage…In most of the experiments, the PAMAM dendrimers were added to the cis-compartment of a bilayer chamber, which was the side of PA63 and C2IIa addition.

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

 Immunization protocols and collection of serum samples:

Cholera antigens (Table 2) were … CT (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 4. Pertussis toxin (PT) from Bordetella pertussis, lyophilized in buffer (List Biological Laboratories, Inc.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 7. Pertussis Toxin, if required (see Table 1; List Biologicals). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Material und Methodik … Ultra Pure LPS (Salmonella minessota R595) List Biological Laboratories, Campbell, USA …

• Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Lung inflammation:

LPS (Escherichia coli 055:B5; List Biological Laboratories, Campbell, CA) in a dose of 20 g in 50 l of PBS was instilled using a modified feeding needle into the trachea of mice. Mice were sedated with isoflurane (Baxter, Deerfield, IL) before instillation. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Sensitization model:

Murine sensitization protocol was similar to previously described one (Kanjarawi et al., 2011). BALB/c mice were intragastrically (ig) sensitized with 5 mg of BLG or CL-BLG with 15 µg of Cholera Toxin (CT, List Biological Laboratories, Inc., CA) on days 0, 7, 14, and 21 (n = 8/group). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction of oral tolerance and immunization:

In some experiments, the mice were immunized intragastrically with 10mg of OVA and 10µg of cholera toxin (List Biological Laboratories, Campbell, CA).

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae