Bacterial Toxin Research Citations

Protein stability:

… For in vitro studies: Stx1 (5 µg/ml) (BEI resources, Cat.#NR-857), Stx2a (BEI resources, Cat.#NR-4478) (5 µg/ml), cholera toxin (5 µg/ml) (List Biological, Cat.#101B) or supernatants were concentrated to a final volume of less …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Toxina Colrica 10mg/ml List Biological Laboraries …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Sensitization of mice:

… For sensitization, Il4raF709 and Il4raF709/IgE-deficient (IgE / ) mice were treated intragastrically with sterile PBS or 250 µg of ovalbumin (OVA; Sigma-Aldrich, St Louis, Mo) plus 20 µg of cholera toxin (List Biological Laboratories, Campbell, Calif) once a week for 3 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry:

After sectioning, free-floating brainstem sections were washed for 30min in a 1:1 pretreatment solution of Triton + PBS (TPBS; 1:200) and hydrogen peroxide. Between each incubation step, sections were rinsed 35 minutes in PBS. Sections were blocked for 1 hour in 10% normal donkey serum (NDS) in PBS. Sections were removed from blocking solution and placed directly into primary antibody for incubation at room temperature (Goat -CTB; List Biologicals, Campbell, CA, USA; 1:40,000 in PBS). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

… phenol – extracted LPS ( Escherichia coli O111:B4) that is free of lipoprotein contamination (List Biological Laboratories); …

• Product #421 –  ULTRA PURE LPS from Escherichia coli O111:B4

Western Blotting:

In Vitro Studies:  Naive wild-type and/or TLR2-/- Ms seeded in 6-well plates (2 x 106 per well) were stimulated with either viable Bb (MOI = 10) or Salmonella typhimurium LPS (200 ng/ml) (List Biological Laboratories). At indicated times post-stimulation, cells were collected and lysed for total protein extracts. …

• Product #225 – LPS from Salmonella typhimurium

Materials and Reagents:

… Lipopolysaccaride (LPS) from Salmonella typhimurium was from List Biological Laboratories (Campbell, CA). …

Culture of Extraplacental Choriodecidual Membranes:

… LTA and LPS treatments were initially dissolved in water and diluted in DMEM/1% FBS. Following acclimation, …

• Product #225 – LPS from Salmonella typhimurium

… the Toll-like receptor [TLR]4 ligand lipopolysacharide [LPS], from Salmonella typhymurium, 0.1 g/mL, List Biological Laboratories (Campbell, CA …

• Product #225 – LPS from Salmonella typhimurium

Protein transfection:

… BMDMs were primed with 10 ng/mL of ultrapure LPS (List Biologicals) for 3 hours to induce pro-IL-1 expression prior to protein transfection, using 88 Profect-P1-lipid based protein delivery reagent (Targeting Systems) as previously described (10, 52); IL-1 secretion was determined by ELISA (Duoset R&D). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents and antibodies:

…LPS was from List Biological Laboratories Inc. (Campbell, CA, USA). …

Real-time reverse transcriptionPCR:

BMDMs or RAW264.7 cells (110⁶ cells ml¹) were cultured up to 8h in the presence or absence of LPS, CpG-DNA, necrotic cells or enzyme-treated necrotic cells. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/