Bacterial Toxin Research Citations

Peptides and proteins:

… Recombinant protective antigen (rPA) was obtained from List Biologicals (Campbell, CA ) ….

 Vaccines and immunization:

… For anthrax and influenza protein vaccines used in Chapter 3, lipids were prepared with S100 lecithin (Lipoid GmBH, Bermany).  These vaccines were delivered via intramuscular injections of 25 L on each the right and left leg.  Each 50 L dose of anthrax vaccine contained 1 g rPA and adjuvants as indicated in figure legends.  …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

PN allergy model including antibody administration:

Intragastric gavage (Delvo, Biel/Bienne, Switzerland) of PN (Kraft, Northfield, IL) with CT (List Biologicals, Campbell, CA) was performed weekly for 4 weeks, with challenge by crude PN extract (Greer, Lenoir, NC) 1 week later.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Antigens and adjuvants: Cholera toxin was from List Biological Laboratories.

Epicutaneous sensitization of mice:

Six- to eight-week-old female mice were anesthetized, and abdominal fur was removed with depilatory cream… SEB or cholera toxin were applied at a dose of 10 µg. In some experiments, antigens were applied to the ear pinnae. Mice were exposed weekly for a total of 6 exposures and challenged a minimum of 1 week after the last exposure.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… and efferent fibers of the IXth and Xth cranial nerves in the central vasculature were visualized using an antibody against CTB (List Biological Laboratories, Cat … CTB, Purified CTB (choleragenoid), List Biologicals, goat, polyclonal, 703, AB_10013220, 1:1,500, (Rosin et al., 2006). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…The primary antibody was goat anti-CTB (List Biological) and the secondary antibody was…

• Product#703 – Anti-Cholera Toxin B Subunit (Goat)

Neurochemical analysis of CTb-labelled boutons in lamina I:

Transverse spinal cord sections from the 3 rats that received only sciatic nerve injections were incubated for 3 days with goat anti-CTb,…Details of the sources and dilutions of the primary antibodies are given in Table 1.

Table 1.

Primary antibodies used in this study.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…MAPKKide was purchased from List Biological Laboratories (Campbell, …

Author did not specify which MAPKKide was utilized.  List Labs provides the following:

• Product #530 – MAPKKide Peptide Substrate (o-Abz/Dnp) for Anthrax Lethal Factor
• Product #531 – MAPKKide Peptide Substrate (DABCYL/FITC) for Anthrax Lethal Factor

EAE induction and scoring:

EAE was induced by s.c. immunization with 200µg MOG_35-55 (MEVGWYRSPFSRVVHLYRNGK) emulsified in 500µg CFA (at 10mg/mL M. tuberculosis H37Ra, Difco) and i.p. injection of 200ng pertussis toxin (List Biological Laboratories, Inc.). Spleen and draining lymph node cells at 13 d post-immunization were taken and stained for flow cytometry analysis. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and Therapeutic Study:

…The PLP-induced model of EAE was used to mimic relapsing-remitting multiple sclerosis (29). Mice were immunized subcutaneously with 200 µg of PLP₁₃₉₁₅₁ in a 0.2 mL emulsion composed of equal volumes of phosphate-buffered saline (PBS) and CFA containing killed Mycobacterium tuberculosis strain H37RA… In addition, 100 ng of pertussis toxin (List Biological Laboratories Inc., Campbell, CA) was injected (100 µL) on two occasions, intraperitoneally on the day of immunization (day 0) and 2 days post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and Therapeutic Study:

Five- to 7-week-old SJL/J female mice were immunized subcutaneously (s.c). with 200 mg of PLP139151 in a 0.2 ml emulsion composed of equal volumes of phosphate-buffered saline (PBS) and complete Freunds adjuvant (CFA) containing killed Mycobacterium tuberculosis strain H37RA (final concentration of 4 mg/ml; Difco). …In addition, 200 ng/100 µL of pertussis toxin (List Biological Laboratories Inc.) was injected intraperitoneally (i.p.) on the day of immunization (day 0) and 2 days post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)