Bacterial Toxin Research Citations

In vivo protocols:

Male C57BL/6J (Jackson Laboratories Bar Harbor) and endothelial NOS deficient (eNOS/) mice (12 and 24 wk old) were anesthetized with 3% isoflurane and exposed to vehicle or 23 mg/kg E. coli endotoxin (E. coli O55:B5, List Biologicals) dissolved in sterile water via supraglottic placement and aspiration. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Reagents:

10 µg/mL cholera toxin (LIST Biological),…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… The SEB challenge model consisted of i.p. injection of 5 x LD₅₀ SEB followed 4 h later by a potentiating dose of lipopolysaccharide (40 g;List Biological Laboratories, Campbell, CA). Protection afforded by c19F1 was dose-dependent with complete survival observed in mice receiving 100 g of c19F1, alone or in combination with c4D7 and cPB10 (Figure 2B). Finally, mAb c4D7 was able to fully protect mice when administered as part of the tripartite cocktail. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… 250 µg of Mycobacterium tuberculosis (Difco). On the same day and again 48 hours later, mice received an intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories). As negative controls, mice were left …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE) induction:

SJL/J mice were injected subcutaneously in the flanks with 200 ml of 1 mM PLP₁₃₉₁₅₁ peptide. The emulsion solution was reconstituted complete Freunds adjuvant (CFA), composed of Freunds incomplete adjuvant (Pierce Biotechnology, Rockford, IL) containing Mycobacterium tuberculosis H37 Ra (2 mg/ml) (Difco Laboratories, Detroit, MI), and PLP_{139 151}. Mice were intravenously injected with 0.2 mg of Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA), in a 100 ml final volume, on days 0 and 2 following sensitization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

BrdU and PTX treatment:

… Mice were treated with 1 mg/ml BrdU (BD) i.v. and administered 1 mg/ml BrdU (Sigma-Aldrich) in drinking water 24 h before analysis. 1 µg PTX (List Bio Labs) was injected i.v. 24 h before analysis. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… On day 0, mice were immunized s.c. with 100 µg MOG35-55 peptide (BEX) emulsified in CFA (Sigma-Aldrich) at the base of the tail. The mice received i.v. 100 ng pertussis toxin (List Laboratories) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin B-subunits, List Biological Laboratories, …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Retrograde tracing experiment:

Rats were anesthetized with a mixture of ketamine …Animals were placed in a Stoelting stereotaxic frame and a hand drill was used to expose the brain surface above the target sites. Iontophoretic injections of 0.5% CTb (List Biologicals, Campbell, CA, USA), which was dissolved in 0.01 M phosphate buffer (PB; pH 7.4), were done by applying a positive current of 1.02.5 A (200 ms pulses at 2 Hz for 15 min) through a chlorinated silver wire placed in a glass pipette (approximately 7µm diameter tip). The coordinates used for injecting CTb were as follows: aPVT at 1.5 mm posterior, 1.0 mm lateral, 5.0 mm ventral, 10 angle toward the middle; and pPVT at 3.0 mm posterior, 0.7 mm lateral, 5.0 mm ventral, 10 angle toward the middle (all coordinates are relative to bregma and the dural surface of the …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

MOG-induced EAE: active induction of EAE

… C57BL/6J and Gas6^/ mice were immunized with MOG_35-55 peptide. MOG_35-…Mice were anesthetized with isoflurane and 100 µl of emulsion was injected subcutaneously on each flank (200 µl total/mouse) on day 0. In addition, 200 µl of pertussis toxin (Ptx, 2.5 µg/ml; List Biological Laboratories) was injected intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)