Bacterial Toxin Research Citations

Plasmids and RNAi:

Additional siRNAs were tested for LGN and CYK4, with a similar impact on anaphase NuMA/p150 Glued localization. Cells were incu- bated with Pertussis toxin (List Biologicals, Lot#181214A1) at 400 ng/ml for 4 h before analysis.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Oral immunization:

Mice received sodium bicarbonate to neutralize gastric acid before oral immunization (9, 22). Thirty minutes later, the mice were immunized orally concurrently with 1 mg OVA (Sigma-Aldrich) and 10 mg cholera toxin (CT; List Biological Laboratories, Campbell, CA). This oral immunization procedure was conducted on days 0, 7, and 14.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Detection of Serum Antibodies to B. pertussis Antigens:

Nunc Maxisorp 96-well plates were coated overnight with 0.2 g/mL pertussis toxin (PT), 0.5 g/mL filamentous hemagglutinin (FHA), or 2 g/mL pertactin (PRN; List Biologicals, Campbell, CA) as described previously…

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Product #170 is currently out of stock.  Large quantities may be obtained through Bulk Requests and Customer Orders.

EAE:

…containing 500 µg of the heat-killed H37Ra strain of Mycobacterium tuberculosis (Difco, Detroit, USA). 200 ng of pertussis toxin (List Biological Laboratories, CA, USA) in 100 µl PBS was administered iv immediately after immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Ovalbumin food allergy model:

The OVA food allergy model has been described previously( 16 , 24 ). Briefly, 6-week-old conventional female BALB/c OlaHsd mice (10–15 animals per group) (Harlan Laboratories) were sensitised via the oral route daily during the first 3 d and then at weekly intervals with 20 mg of OVA (Sigma) along with 10 μg/mouse of cholera toxin used as an adjuvant (List Biological Laboratories) for a continuous period of 7 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Analysis of T-cell and monocyte population activation:

…Monocyte populations in the PBMC samples (200 l per well) were stimulated in separate ninety-six-well plates with 10 g/l of ultra-pure lipopolysaccharide (LPS; List Biological Laboratories, Inc.) per well or an equal volume of sterile endotoxin-free water as a negative control. PBMC were incubated at 37°C in 5 % CO₂ and the supernatants were removed after 24, 48 and 72 h for the analysis of monocyte-derived cytokines as described below.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Memory B Cell ELISPOT:

… Cultured PBMCs were washed then plated at 1:2 serial dilutions (3.75 10⁵ 1.6 10⁴ cells/well) in triplicate onto ELISPOT plates coated with 10 g/mL PA (List Biologicals, Campbell, CA, USA) … The frequency of PA-specific ASCs was defined as: [(anti-PA IgG spots per 100,000 input cells)/(total IgG spots per 100,000 input cells)] 100.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Cell Treatment:  

The cells were incubated in HDMEM + 10% FBS for 72 hours before they were
treated with either media (control), 1M CP 55,940 (Tocris; Bristol, UK), 100pg/mL,
1ng/mL, 10ng/mL, or 20ng/mL E. coli O55:B5 LPS (List Biological Laboratories, Inc.;
Campbell, CA),

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Reagents:  the receptorbinding protein protective antigen and metalloprotease lethal factor were from List Biological

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

SNAPtide Assay[44]

Recombinant 425aa BoNT/A LC was used throughout all assays and was prepared as previously described.[45] All SNAPtide assays were run in 40 mM HEPES + 0.1% Triton X-100 at pH 7.4. BoNT/A LC concentrations were 18.5 nM while SNAPtide (prod. no. 521, List Labs) concentrations were 5 M. Fluorescence was recorded continuously for 1.5 h to calculate an accurate k_obs using equation 1. In the preincubation experiments, inhibitors at the specified concentrations were incubated with 925 nM enzyme and diluted 50-fold into substrate and initial rates of SNAPtide cleavage were measured. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin