Bacterial Toxin Research Citations

Materials:

…and pertussis-toxin from List Biological Laboratories (Campbell, CA, USA). …

Pertussis toxin inhibits mH₄R- but not mH₁R-mediated effects:

Experiments with pertussis toxin (PTX), which selectively inhibits G_i-protein activation by GPCRs [41], [42], were performed. HEK293 mH₁R and HEK293 mH₄R cells were incubated with or without PTX for 18 h and then the [Ca²⁺]_i and the forskolin-induced cAMP concentrations were evaluated after stimulation with HA. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Protocols:

All methods have been described recently. [8] In brief, studies were performed in male C57Bl/6 mice weighing 2530 g (Charles River Laboratories). Mice were injected i.p. with LPS (Escherichia coli 055, LPS no. B5, List Biological Laboratories, Campbell, CA) at a dose of 10 mg/kg (volume diluted with saline in 0.5 ml) once a week for 14 weeks. Mice were injected with 0.5 ml of saline as a control. Mice were monitored daily…

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

EAE Induction and in Vivo Treatments:

EAE was induced as described (16) with minor modifications. Eight to twelve-week-old mice were actively immunized with 100 µl of 1 mg/ml MOG(3555) peptide… At immunization and 48 h later, all mice received 500 ng of pertussis toxin (List Biological Laboratories) intraperitoneally.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis:

The mice received three daily intraperitoneal (i.p.) administrations of 500 ng of pertussis toxin (PT) (List Biological Laboratories) starting on day 1. The disease severity was scored as previously described (Miyake et al., 2013). For the in vitro restimulation analysis, cells were collected from the axillary, inguinal, and lumbar (paraaortic) lymph nodes on day 23.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tetanus toxoid stimulation assay:

Total PBMCs from healthy donors were thawed in RPMI-1640 medium containing 10% heat-inactivated human serum (GemCell). Cells were washed twice with medium and suspended at a final concentration of 1.5 10⁶ cells/ml. Tetanus toxoid (List Biological Laboratories) was added at a concentration of 2.5g/ml. …

• Product #191 – Tetanus Toxoid from Clostridium tetani

EAE induction – C57BL/6J mice:

EAE was induced in ~5 week old C57BL/6J female mice (Jackson Laboratory). … Once completed, an intraperitoneal (i.p.) injection of pertussis toxin (PTX; 100 ng/100µl saline; List Biological Laboratories, Campbell, CA) was administered. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Primary Human Monocytes:

…Cells were allowed to adhere to the plates in an incubator at 37°C, 5% CO₂, and 95% relative humidity for 2 hrs. After 2 hrs, the cell culture medium was aspirated, cells were washed with fresh medium and incubated with 5 ng/mL of ultrapure LPS from Escherichia coli O111:B4 (List Biological Laboratories, Inc., Campbell, CA) over night for priming. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Materials:

… Lipopolysaccharides from Salmonella minnesota R595 (Re) were purchased from List Biological Laboratories Inc. (Campbell, CA).  Biotinylation of S. minnesota R595 (Re) lipopolysaccharides was performed using EZ-Link Sulfo-NHS-LC-Biotin…

Binding Assay to Lipopolysaccharide-immobilized Sepharose:

Sepharose coupled to streptavidin was incubated for 2 h at 4 C in the presence or absence of 10 g of biotinylated lipopolysaccharides in 1.0 ml of TBS1, and Sepharose, obtained by centrifugation at 6,000 g for 5 min was washed with TBS1. …

Amidase Activity of Recombinant Proteins Activated by Lipopolysaccharides:

Recombinant proteins were incubated in the presence of various concentrations of lipopolysaccharides in 100 mm Tris-HCl, pH 8.0, at 37 C for 20 min. …

Lipopolysaccharide-binding Assay with a Surface Plasmon Sensor:

Biotinylated lipopolysaccharides (2.3 m in 10 mm Hepes-NaOH, pH 7.4, containing 150 mm NaCl) were immobilized on a sensor chip SA of a BIAcore X system…

• Product #304 – LPS from Salmonella minnesota R595 (Re)

… For regulatory T cell depletion, wild-type control or Foxp3-DTR mice were injected intraperitoneally with 1.5 g of DT (Calbiochem, San Diego, CA, USA or List Biological Laboratory, Campbell, CA, USA) on days 0, 1, 4, and 7 and examined on day 8. CD4+, CD25+, Foxp3+ …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

(Transwell Migration Assays) – Pertussis toxin (PTX) was purchased from List Biological Laboratories, Inc. (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)