Bacterial Toxin Research Citations

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA), …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… Purity and characteristics of the Purified C Fragment were determined by HPLC and Western Blot, in comparison with Standard C Fragment purchased from List Biological Laboratories Inc., USA. …

• Product #193 – Tetanus Toxin C-Fragment from Clostridium tetani

TNA Assay:

The TNA Assay measures the levels of anthrax lethal toxin neutralizing antibody using an in vitro cytotoxicity assay. The assay was originally validated at the CDC, but was then transferred and validated at Battelle, where the testing of these serum samples occurred[8, 9]. Briefly, microtiter cell plates were seeded with J774A.1 cells and allowed to adhere. In separate microplates a mixture of recombinant protective antigen (rPA, List Biological Laboratories, Inc., Campbell, California, Cat. No. 171B) and recombinant lethal factor (rLF, List Biological Laboratories, Inc., Campbell, California Cat. No. 172B) was added to serial dilutions of the test samples and controls and incubated prior to transfer to the cell plate. The final concentration of rPA was 0.05 g/mL and the final concentration of rLF was 0.04 g/mL. MTT was then added to the cell plates to allow viable cells to reduce the MTT dye. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis
• Product #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis

Induction of EAE:

…Female SJL/J mice were immunized by subcutaneous injection into the flank regions with emulsified PLP139-151 (50 nmol/mouse). Pertussis toxin (List Biological Laboratories, Campbell, CA, USA) dissolved in PBS was injected intravenously (100 ng/mouse/day) on the day of immunization and 2 days later. Mice immunized by subcutaneous injection of emulsion without PLP139-151 served as normal controls. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Please note that a goat anti-CTB antibody is also available from List Biological Labs and can be then used as an alternative to the rabbit anti-CTB antibody. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

… And pertussis toxin 200 ng (List Biological laboratories, USA) on the day of immunization and on the second day after immunization. After immunization, daily weight and clinical symptoms of the mice were measured. The progress of the EAE is …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…Shiga toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Shiga toxin was utilized.  List Labs provides Product #161 – Shiga Toxin 1 from Escherichia coli and Product #162 – Shiga Toxin 2 from Escherichia coli.

EAE induction and treatments:

EAE was induced by immunization female mice with MOG₃₅₅₅ peptide emulsified in CFA (Difco Laboratories) at a dose of 100µg (C57BL/6 and F1 mice) or 150µg (NOD mice) per mouse, followed by the administration of Pertussis toxin (150 ng per mouse; List Biological Laboratories, Inc.) on days 0 and 2 as described.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Preparation of primary mouse corneal epithelial cells:

We followed the methods described previously to conduct the primary culture of the corneal epithelial cells by corneal discs obtained from freshly killed Kcnj10^/ (KO; 710 days old) and the corresponding wild-type littermates… The pellet cells were resuspended with Epilife…with 100 ng/ml cholera toxin (List Biological Laboratories, Campbell, CA) and 1 antibiotic. The subcultured primary cells at passage 2 were used for electrophysiology studies. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Stimulants of ROS production included PMA (Sigma-Aldrich) at 10 7 M, Salmonella typhimurium LPS (List Biologicals Laboratories, Inc., Campbell, CA) at 1 g/ml, …

• Product #225 – LPS from Salmonella typhimurium