Bacterial Toxin Research Citations

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA), and it was dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Sensitization and treatment protocol:

…Sensitization began when mice were 6 to 12 weeks old. Sensitized mice received, by gavage, a mixture of PT-gliadin (500 µg) and cholera toxin (CT) (25 µg) (List Biological Laboratories Inc.) once a week for 3 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For the experiment, the TA muscle on the immobilized side was injected with either 1 l saline (N = 5), or 1 ng tetanus toxin dissolved in 1 l saline (N = 7; List Biological Laboratories) at the time of joint fixation. …

• Product #190 – Tetanus Toxin from Clostridium tetani

Peptides and proteins:

… Recombinant protective antigen (rPA) was obtained from List Biologicals (Campbell, CA ) ….

 Vaccines and immunization:

… For anthrax and influenza protein vaccines used in Chapter 3, lipids were prepared with S100 lecithin (Lipoid GmBH, Bermany).  These vaccines were delivered via intramuscular injections of 25 L on each the right and left leg.  Each 50 L dose of anthrax vaccine contained 1 g rPA and adjuvants as indicated in figure legends.  …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

PN allergy model including antibody administration:

Intragastric gavage (Delvo, Biel/Bienne, Switzerland) of PN (Kraft, Northfield, IL) with CT (List Biologicals, Campbell, CA) was performed weekly for 4 weeks, with challenge by crude PN extract (Greer, Lenoir, NC) 1 week later.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Antigens and adjuvants: Cholera toxin was from List Biological Laboratories.

Epicutaneous sensitization of mice:

Six- to eight-week-old female mice were anesthetized, and abdominal fur was removed with depilatory cream… SEB or cholera toxin were applied at a dose of 10 µg. In some experiments, antigens were applied to the ear pinnae. Mice were exposed weekly for a total of 6 exposures and challenged a minimum of 1 week after the last exposure.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… and efferent fibers of the IXth and Xth cranial nerves in the central vasculature were visualized using an antibody against CTB (List Biological Laboratories, Cat … CTB, Purified CTB (choleragenoid), List Biologicals, goat, polyclonal, 703, AB_10013220, 1:1,500, (Rosin et al., 2006). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…The primary antibody was goat anti-CTB (List Biological) and the secondary antibody was…

• Product#703 – Anti-Cholera Toxin B Subunit (Goat)

Neurochemical analysis of CTb-labelled boutons in lamina I:

Transverse spinal cord sections from the 3 rats that received only sciatic nerve injections were incubated for 3 days with goat anti-CTb,…Details of the sources and dilutions of the primary antibodies are given in Table 1.

Table 1.

Primary antibodies used in this study.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

…MAPKKide was purchased from List Biological Laboratories (Campbell, …

Author did not specify which MAPKKide was utilized.  List Labs provides the following:

• Product #530 – MAPKKide Peptide Substrate (o-Abz/Dnp) for Anthrax Lethal Factor
• Product #531 – MAPKKide Peptide Substrate (DABCYL/FITC) for Anthrax Lethal Factor