Bacterial Toxin Research Citations

… Cells were stimulated with 10 ng/ml ultra pure lipopolysaccharide (LPS; Salmonella minnesota R595, List biological laboratories, CA),…

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Reagent:  LPS from Salmonella Minnesota R595 was from List Biological Laboratories.

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Bactericidal assays:

… Competition with LPS was carried out as described previously (4, 5) by preincubating serum or plasma samples for 60 min at room temperature with 100 g/ml of purified LPS from S. Typhimurium (List Biological Laboratories, Campbell, CA). …

• Product #225 – LPS from Salmonella typhimurium

Chapter 2 – Cooperation between classical and alternative NF-B pathways regulates pro
inflammatory responses in epithelial cells

Mice: C57Bl/6 mice were anesthetized and given 5g LPS oropharyngeally
(List Biological Laboratories, Inc., Campbell, CA) and 24 h later, euthanized by a lethal
pentobarbital injection. …

Chapter 4 – Glutathione S-Transferase pi modulates Nuclear Factor-kappaB activation and pro
inflammatory responses in lung epithelial cells 

Cell Culture and reagents:  C10 cells were starved in medium containing 0.5% FBS for 16
hours prior to exposure of 1g/mL Lipopolysacharide (LPS, List Biological Laboratories,
Inc).  …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

LPS-induced TNF- release ex vivo:

Samples of EDTA blood (Cohort II) were stimulated with 1 ng/ml LPS (List Laboratories, Campbell, CA, USA) in Modified Eagle Medium (Invitrogen, Grand Island, NY, USA) control or medium only, and incubated in pyrogen-free 1.5-ml tubes for 4 h at 37C, rotating end-over-end, prior to collection of the extracellular medium for subsequent TNF- ELISA (R&D Systems, Minneapolis, MN, USA or BD BioSciences, San Jose, CA, USA).

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents:

… LPS from Escherichia coli O111:B4 was obtained from List Biological Laboratories (Campbell, CA, USA). …

ELISAs:

Isolated BMCs were cultured in the presence of M-CSF (20 ng/ml) + G-CSF (10 ng/ml). On Day 5, cells were sorted using a FACSAria III cell sorter, washed with PBS twice, and cultured in a 96-well plate for 2 h. Subsequently, cells were treated with 100 ng/ml LPS, and cytokine levels were determined in cell culture supernatants using commercially available quantitative ELISA kits from eBioscience. …

• Product #201 – LPS from Escherichia coli O111:B4

Mice:

… Mice were injected with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitoneally on the day of immunization and 2 days after immunization. Mice were assessed daily for clinical signs of EAE according to the following scale designated as EAE clinical score: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Control mice were injected sc bilaterally with an emulsion of PBS, 8 mg/mL H37RA and incomplete Freund’s adjuvant. 100 ng Pertussis toxin (List Biological Laboratories, Inc, Campbell, CA, USA) was administered ip on days 0, 1 and 2 to induce EAE. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

For EAE-immunized mice, the adjuvant pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA)was injected intravenously on the day of immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental allergic encephalomyelitis, evaluation of symptoms, and administration of peptides:

…On day 1, SJL/J mice were injected with 300µg/mouse bovine myelin basic protein (Invitrogen) that was emulsified in Complete Freund’s Adjuvant with…and injected subcutaneously into 2 sites at the base of the tail along with 400ng/mouse of pertussis toxin (List Biological Laboratories, Inc.) in PBS i.p… On day 3, the pertussis toxin injection was repeated.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)