Bacterial Toxin Research Citations

Experimental Autoimmune Encephalitis:

Mice were immunized with 50 µg of MOG peptide (myelin oligodendrocyte glycoprotein amino acids 3555) emulsified in IFA containing 50 µg Mycobacterium tuberculosis (strain H37RA) by subcutaneous injection. At the time of immunization and three days later, mice received 300 ng of pertussis toxin (List Biological Laboratories) intravenously. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adoptive Transfer Experiments:

Three groups of mice were infected with ACA (110³) and after 15 days, the animals were sacrificed and cell suspensions were prepared from lymphoid tissues as above. We used two protocols to generate primary T cell cultures: (i) cells were stimulated with concanavalin-A (con-A, 1 g/ml) for two days. …Pertussis toxin (PT; 100 ng; List Biological laboratories, Campbell, CA) was administered i.p. on days 0 and 2 posttransfer, and the animals were monitored for clinical disease. At termination, animals were euthanized, and CNS tissues were collected for histology [16][18].

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

General reagents:

…Anthrax PA83 was purchased from List Biological Laboratories (Campbell, CA, USA). …

MPII proteolysis of proteins in vitro:

Anthrax PA83 (2 g; ~ 1 m) and the following purified human proteins the furin 27107 prodomain (1.8 g, ~ 9 m) [20], the MT1MMP proenzyme (2 g; ~ 2 m), MBP (2 g; ~ 5 m) and BTN3A2 (2 g; ~ 2.5 m) were each coincubated for 1 h at 37 C with increasing concentrations of MPII in 50 mm Hepes, pH 8.0, containing 1 mm CaCl₂, 0.5 mm MgCl₂ and 10 m ZnCl₂. As a control, PA83 and the MT1MMP proenzyme were coincubated for 1 h at 37 C with furin (at the 1 : 100 to 1 : 10 000 enzyme/substrate molar ratio) in 20-L reactions containing 100 mm Hepes, pH 7.5 and 1 mm CaCl₂. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Keratinocyte culture medium:

KCM was composed from a basal mixture of three parts Dulbecco’s modified Eagle’s medium (DMEM; Sigma Aldrich, St. Louis, MO) and one part Nutrient Mixture F-12 Ham (Sigma Aldrich) supplemented with …1 nmol/L cholera toxin (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Analysis of lipid rafts:

… For analysis of lipid rafts, cells were incubated for 15 min with 10 g/mL cholera toxin B (CTB) (List Biologicals) in ice-cold DMEM:F12 with 1% of FBS at 4C and then with goat anti-CTB (1:1,000; List Biologicals) for 15 min at 4C. CTB binds the ganglioside GM1,…

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid)
from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Endopeptidase assay:

BoNT/B LC (LCB) was assayed for endopeptidase activity by using Vamptide (OBZ/DNP; List Biological, Campbell, CA) as its substrate. This peptide is intramolecularly quenched by FRET (fluorescence resonance energy transfer). …

• Product #540 – VAMPtide Peptide Substrate (o-Abz/Dnp) Substrate for C. botulinum Type B Neurotoxin

Chemicals and reagents were purchased from: … phenol-extracted LPS (Escherichia coli O111:B4) free of lipoprotein contamination (List Biological Laboratories); …

Microparticle isolation and quantitation:

Washed platelets (109/ml) were pretreated with 30 M MG132, 40 M
bortezomib or buffer for 30 min before an overnight treatment with thrombin (0.2
U), ADP (100 M) or 0.1 g/ml LPS with addition of 0.1 g/ml each of human
recombinant CD14 and LPS binding protein (LBP). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Use of RNAi in mouse macrophages to test candidate genes:

… Twenty-four hours after transfection, cells were incubated in the presence of 20 ng/ml LPS (List Biological Labs), and supernatants were collected for ELISA (R&D Systems). …

Overexpression studies in mouse macrophages:

… Twenty-four hours after transfection, cells were stimulated with LPS for 6 hr and then luciferase activity was monitored using the Dual luciferase assay kit (Promega) using SV40-rluc as a normalization control for transfection efficiency. …

Author has specified that the following LPS product was used:

• Product #201 – LPS from Escherichia coli O111:B4

EAE Induction and clinical evaluation:

For determining whether the bacterial expressed peptides similar to MOG could induced
active EAE in B6 mice (Figure 5F), mice were immunized subcutaneously with 100 µg
of MOG3949 or 11 amino acid versions of homologous foreign peptides emulsified in
CFA containing 4 mg/ml Mycobacterium tuberculosis. In addition, the mice received 200
ng of pertussis toxin (List Biological Laboratories) intravenously on the day of, and 2
days after, peptide immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PTX was from List Biological Laboratories (Campbell, CA, USA). … 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)