Bacterial Toxin Research Citations

Enzyme-linked immunosorbent assay (ELISA): 

An ELISA was used to measure the levels of IgG against the toxins and bacterin. Blood samples were collected before (pre-immune) immunization as well as 11 and 17 weeks after the first immunization. Purified TcdA (List Biological Laboratories, USA), …

• Product #152C – Toxin A from Clostridium difficile

Reagents:

LPS (cat. no. 421),…were purchased from List Biological Laboratories (Campbell, CA),…

Generation of stable control (nontargeting) and MyD88 and TLR2 knockdown RAW 264.7 cells:

…Functional knockdown was assessed by using known agonists (LPS and Pam3CSK4) followed by measurement of COX-2 protein induction and/or agonist-mediated cytokine secretion. Two cell pools with the greatest degree of knockdown as well as the NT cell pool were used in the acylcarnitine treatment experiments.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Experimental autoimmune encephalomyelitis:

The procedure to induce EAE was performed according to the instructions. …Pertussis toxin (200 ng per mouse; List Biological Laboratories) in PBS was administered i.v. on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

Mice (78 weeks) were immunized subcutaneously with 50 or 200 µg of myelin oligodendrocyte glycoprotein (MOG₃₅₅₅; MEVGWYRSPFSRVVHLYRNGK)… Pertussis toxin (List Biological Laboratories, Campbell, CA) in phosphate-buffered saline (PBS; at 200 ng/mouse) was administered intraperitoneally on days of immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

…For active immunization, B6 mice were immunized subcutaneously in both sides of the flank with a total dose of 200 µg MP4 in CFA. Pertussis toxin (List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Quantification of RGC survival after injury:

RGC number was examined after 14 days since ONC. We used the anterograde tracer FITC-conjugated cholera toxin B (CTB-FITC; List Biological, Campbell, CA) to inject into the vitreous chamber of the eye two days before fixation and removal of the eyeball. …

• Product #106 – Cholera Toxin B Subunit (CTB) from Vibrio cholerae FITC, Conjugate

Induction of EAE and anti-VLA-4 mAb treatment:

…EAE was induced by the subcutaneous immunization of 7-week-old C57BL/6 mice with 200 µl emulsions of 200 µg MOG_35-55 peptide (MEVGWYRSPFSRVVHLYRNGK; Protein and Nucleic Acid Facility, Stanford University, Stanford, CA) in Complete Freund’s Adjuvant (4 mg/ml). Additionally, animals received an i.p. injection of 100 µg pertussis toxin (List Biologicals, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunohistochemistry:

Immunohistochemistry was performed on 4 rats injected with the retrograde tracer Cholera toxin B fragment (CtB, List Biologicals, Campbell CA, USA) to identify phrenic motor neurons in the cervical spinal cord. Each rat received an intrapleural injection of CtB for retrograde labeling of spinal phrenic motor neurons using a technique similar to that described previously…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Intranasal vaccination:

… Mice were rested for 4 hours to allow gene expression to peak and subsequently administered with 1µg cholera toxin (CT, List Biologicals) in 10µl PBS. …In summary, each mouse received a total of 9µg of OVA (or GFP) mRNA nanoparticles and 1µg of CT per vaccination.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry and image acquisition:

Primary Antibodies – Goat List 703 1:2000, List Biological Labs

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)