Bacterial Toxin Research Citations

… Briefly, BALB/c mice were injected three times intraperitoneally with 100 or 150 µl of the viral suspension (1.3 10 10 copies/ml) in PBS on days 0, 14 and 53 and with 0.1 µg of inactivated pertussis toxin (Pertussis Toxin Salt-Free; List Biological Laboratories, Inc., Campbell, CA …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Toxin injection:

Mice were injected intraperitoneally with PTX (20 µg/kg), PTX-A (5.3 µg/kg), PTX-B (14.5 µg/kg), PTX^mut (20 µg/kg), … The toxins were purchased from List Biological Laboratories, … In most experiments, mice were given a single injection and killed 3 or 6 h later. In indicated experiments, they were given 2 injections, 2 days apart, and killed 24 h after the last injection.

Bone marrow transplantation:

… Chimeric mice were injected with PTX 2 months after transplantation.

EAE induction by active immunization:

Mice were subcutaneously injected into both flanks with a total of 200 µl of emulsion containing 300 µg of MOG peptide 3555 (Feldan) dissolved in saline and mixed with an equal volume of complete Freund’s adjuvant containing 500 µg of killed Mycobacterium tuberculosis H37 RA (Difco Laboratories). The mice were also injected intraperitoneally with 20 µg/kg of PTX immediately and 2 days after immunization.

Ex vivo stimulation of leukocytes:

Peritoneal leukocytes were harvested by flushing the peritoneum of naive mice with 5 ml D-PBS. Cells were pooled in DMEM supplemented with 2% FBS, transferred in 24-well plates at a density of 210⁶ cells per well in 500 µl containing or not 500 ng/ml PTX, PTXmut or heat-inactivated PTX (heated at 80C for 30 min). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

Mice were injected subcutaneously in both flanks with 200 µL of inoculum containing 100 µg MOG₃₅₅₅ and 0.5 mg Mycobacterium tuberculosis H37Ra (Difco Laboratories, Detroit, MI) in incomplete Freund’s adjuvant. Pertussis toxin (200 ng; List Biological Laboratories Inc., Campbell, CA) was injected intravenously on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… The animals also received 150 ng of pertussis toxin (PT) (List Biological Laboratories) intra-peritoneally (IP) on day 0 and day 2. The animals were followed daily, and clinical signs of EAE were assessed on a daily basis according to the following scores: 0, no disease; 1, loss …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE in DR2-Tg and MBP-TCR/DR2-Tg mice

…In addition, mice were given pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA) on days 0 and 2 postimmunization (75 and 200 ng/mouse, respectively).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

The mice also received two 400 ng intraperitoneal injections of pertussis toxin (List Biological Laboratories,… immediately and 48 h later in 100 µL of phosphate buffered saline (PBS).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

In addition, mice were treated with pertussis toxin (PTX; 200 ng, List Biological Laboratories) administered intraperitoneally on the day of immunization (day 0) and on dpi 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE disease models:

On days 0 and 2, 50 µg pertussis toxin (List Biological Laboratories) was given i.v. Subsequently, these mice were treated with KN-93 or PBS twice a week.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vivo experiments

EAE was induced by subcutaneous injection of 150 µg MOG peptide (amino acids 3555), in 0.1 ml of CFA emulsion containing 400 µg Mycobacterium tuberculosis (Difco), into wild-type or Traf5^/ B6 mice on day 0. The mice received intraperitoneal injection of 200 ng pertussis toxin (List Biological Laboratories) on days 0 and 2. Clinical signs of EAE were assessed according to the following…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The nasal tissue-derived cells were immunostained without in vitro culture or stimulation. The frozen PBMCs were thawed for phenotype analysis after stimulation with SEB (List Biological Laboratories Inc., Campbell, CA) in 96-well round-bottom plates. …