Bacterial Toxin Research Citations

Keratinocyte culture medium:

KCM was composed from a basal mixture of three parts Dulbecco’s modified Eagle’s medium (DMEM; Sigma Aldrich, St. Louis, MO) and one part Nutrient Mixture F-12 Ham (Sigma Aldrich) supplemented with …1 nmol/L cholera toxin (List Biological Laboratories, Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Analysis of lipid rafts:

… For analysis of lipid rafts, cells were incubated for 15 min with 10 g/mL cholera toxin B (CTB) (List Biologicals) in ice-cold DMEM:F12 with 1% of FBS at 4C and then with goat anti-CTB (1:1,000; List Biologicals) for 15 min at 4C. CTB binds the ganglioside GM1,…

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid)
from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Endopeptidase assay:

BoNT/B LC (LCB) was assayed for endopeptidase activity by using Vamptide (OBZ/DNP; List Biological, Campbell, CA) as its substrate. This peptide is intramolecularly quenched by FRET (fluorescence resonance energy transfer). …

• Product #540 – VAMPtide Peptide Substrate (o-Abz/Dnp) Substrate for C. botulinum Type B Neurotoxin

Chemicals and reagents were purchased from: … phenol-extracted LPS (Escherichia coli O111:B4) free of lipoprotein contamination (List Biological Laboratories); …

Microparticle isolation and quantitation:

Washed platelets (109/ml) were pretreated with 30 M MG132, 40 M
bortezomib or buffer for 30 min before an overnight treatment with thrombin (0.2
U), ADP (100 M) or 0.1 g/ml LPS with addition of 0.1 g/ml each of human
recombinant CD14 and LPS binding protein (LBP). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Use of RNAi in mouse macrophages to test candidate genes:

… Twenty-four hours after transfection, cells were incubated in the presence of 20 ng/ml LPS (List Biological Labs), and supernatants were collected for ELISA (R&D Systems). …

Overexpression studies in mouse macrophages:

… Twenty-four hours after transfection, cells were stimulated with LPS for 6 hr and then luciferase activity was monitored using the Dual luciferase assay kit (Promega) using SV40-rluc as a normalization control for transfection efficiency. …

Author has specified that the following LPS product was used:

• Product #201 – LPS from Escherichia coli O111:B4

EAE Induction and clinical evaluation:

For determining whether the bacterial expressed peptides similar to MOG could induced
active EAE in B6 mice (Figure 5F), mice were immunized subcutaneously with 100 µg
of MOG3949 or 11 amino acid versions of homologous foreign peptides emulsified in
CFA containing 4 mg/ml Mycobacterium tuberculosis. In addition, the mice received 200
ng of pertussis toxin (List Biological Laboratories) intravenously on the day of, and 2
days after, peptide immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PTX was from List Biological Laboratories (Campbell, CA, USA). … 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… This was injected into both flanks and the base of the tail, in a total volume of 250 µl, immediately followed by intravenous injection of 300 ng pertussis toxin (List Biological, Campbell, CA) in 0.1 ml PBS. An identical pertussis toxin injection was repeated 3 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

On the day of immunization (day 0) and 48 h later (day 2), 200 ng pertussis toxin (List Biological laboratories Inc. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

Mice were subcutaneously immunized with 200 µg MOG_35-55 peptide emulsified in a equal volume of complete Freunds adjuvant…followed by two i.p. injections of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) 48 h apart. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)