Citations

Cytotoxicity studies:  Recombinant B. anthracis lethal factor was purchased from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Lethal Factor was utilized.  List Labs offers Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

… STZ is a widely used and accepted model of type 1 diabetes despite its nonspecific tissue toxicity (8, 9). DT or mutant DT (List Biological Laboratories) was injected intraperitoneally at the dose of 25 ng/g body wt weekly for 6 wk beginning 1 wk after STZ injection. …

• Product #149A – Diphtheria Toxin CRM197, Mutant
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… We injected mice intraperitoneally with … purified B. pertussis toxin (List Biologicals) on days 0, 3, and 7 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… C57BL/6J mice received 200 ng pertussis toxin (List Biological Laboratories, Hornby, ON, Canada) in 500 µl sterile phosphate-buffered saline (PBS) on the day of immunization and 48 h later. Clinical symptoms …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Ex vivo activation of peripheral blood mononuclear cells:

…For the activation of monocytes, 200 l PBMC were stimulated in separate wells (ninety-six-well plates) with 10 g/l of ultra-pure lipopolysaccharide (LPS; List Biological Laboratories, Inc.) or an equal volume of sterile endotoxin-free water as a negative control. PBMC were incubated at 37C in 5 % CO₂ and supernatants were removed after 24, 48 and 72 h for the analysis of monocyte cytokine production as described below.

Author has informed that the following LPS was utilized:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Mice:

Tumors that were 69 mm in diameter were selected, and volumes were monitored by high-resolution ultrasound as detailed in… KPC (DTR transgene) mice were treated every 48 h with 25 ng/g of DTx (List Biologicals) in PBS,…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

… Cholera toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

[32P]ADP-ribosylation Assays
ADP-ribosylation was performed essentially as described (33), with minor modifications. Briefly, cholera toxin was activated at a concentration of 2 mg/ml by incubation for 30 min at 30 °C in media containing 100 mm DTT, 10 mg/ml BSA, 1% SDS, and 200 mm HEPES, pH 7.5. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Anatomical Tracing:

…Retrograde labeling of long propriospinal interneurons was carried out by injecting 1 µl of Cholera Toxin Subunit B (CTB; 1%; List Biological) into each side of the lumbar spinal cord (level L2 L3) using a glass micropipette …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

Purified LPS was purchased from List Biologicals (San Jose, CA, USA) …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Immuno-assays:

…Plates were frozen at -80C until the day of use when they were thawed to 37C. 380 l of fresh whole blood anticoagulated with citrate from each subject was mixed 1:1 with RPMI media and added to each well [23]. Final concentrations of stimuli were: …E. coli O111:B4 LPS 10 ng/ml (List Biologicals), and S. minnesota Re595 LPS 10 ng/ml (List Biologicals). Plates were placed on a shaker at 37C under 5% CO₂ for 6 hours before begun spun down and the plasma supernatant removed and frozen at -80C. …

• Product #201 – LPS from Escherichia coli O111:B4
• Product #304 – LPS from Salmonella minnesota R595 (Re)