Citations

Solutions and Chemicals:

Bordetella pertussis holotoxin (PTX) was purchased from List Biological Laboratories.

Coupling of FFA3 to N-type Ca²⁺ channels in sympathetic neurons:

Expression of FFA3 followed by C2 or C3 application produced I_Ca inhibition typical of G_i/o-coupled GPCRs, which was rapid in both onset and washout, voltage-dependent, and greatly reduced by PTX pretreatment…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In experiment 3, a lateral incision of the neck skin was performed. Then, in seven rats the SLN of the left vagus nerve proximal to the point passing through the thyrohyoid membrane was dissected free from surrounding tissues, and cut. In nine other rats, the left GpN-ct distal to the branching point of the carotid sinus nerve was dissected free from surrounding tissues, and cut. The central cut end of these nerves was inserted into a piece of polyethylene cup (inner diameter: 1.01.5 mm) which was filled with 0.2 µl of 1% CTb (List Biological Laboratories, Campbell, CA) dissolved in 0.1 M phosphate buffer; the space between the inserted nerve and the opening of the cap was sealed with vaseline and/or dental wax…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Botulinum neurotoxin B light chain (List Biological Laboratories) was dissolved to a final concentration of 200M in deionized water purified to a final resistance of 16.7M. …

• Product #620A – Botulinum Neurotoxin Type B Light Chain, Recombinant

… LPS (Escherichia coli 055: B5) conjugated with fluorescein isothiocyanate (FITC-LPS, molar ratio 24: 1) was obtained from List Biological Laboratories, Inc.(Campbell, CA). …

List Labs does not provide LPS (Escherichia coli 055: B5) conjugated with fluorescein isothiocyanate (FITC-LPS); however, the following LPS is available for purchase:

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

MATERIALS AND METHODS:

Lipopolysaccharide Escherichia coli 055: B5 lipopolysaccharide (LPS)(List Biologicals, Campbel1, CA, USA) was used after extraction of free lipid by hexane: 2-pro- pano. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

… B. anthracis rPA protein was from List Biological Laboratories, Inc. (Campbell, CA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Mice were sensitized intragastrically (ig) using a blunt needle with 6 mg PE or 20 mg whey in 0.5 mL PBS with 10 µg cholera toxin (CT; List Biological Laboratories Inc, Campbell, CA, USA) as an adjuvant while sham mice received CT only (10 µg/0.5 mL PBS). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell Culture:

… 100 ng/ml cholera toxin (List Biological Labs; Campbell, CA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… The cells were resuspended in cKSFM which is kera tinocyte serum-free medium (KSFM), supplemented with 50 g/ml bovine pituitary extract (BPE), 5 ng/ml human recombinant epidermal growth factor (EGF, all Gibco), and 30 ng/ml cholera toxin (LIST Biological Laboratories Inc.). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Strains and animals:

…Some mice were intranasally administered 1 µg of cholera toxin (CT; List Biological Laboratories, Compel, CA) 4 or 5 weeks prior to challenge, because these mice were controls in other experiments in which the experimental mice received immunizations using CT as an adjuvant. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae