Bacterial Toxin Research Citations

2.4.4 Purification of PA and LF

PA83 was expressed and purified by the Barth group. After purification, PA83 was activated in vitro to PA63 and stored at -80 °C.
LF was purchased from List Biological Laboratories (see 2.1.7) and stored at -80 °C.

Author did not specify which Lethal Factor (LF) was utilized; List Labs provides the following LF:

• Prodcut #172 – Anthrax Lethal Factor (LF), Recombinant from B. anthracis
• Product #169L – Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence

2.1.2 Study II: Examination of the contribution of neuroinflammatory processes such as
BBB leakage and focal inflammation to changes in mechanical properties

For in vivo MRE and MRI experiments adoptive transfer EAE was induced in 21 SJL mice (female, 9-12 weeks, Janvier, SAS, France, registration number G106/19) by the transfer of myelin proteolipid protein (PLP)-reactive lymphocytes. SJL mice were used as the animal strain of choice to induce EAE (active and adoptive transfer), as they display a high disease susceptibility after induction. PLP-reactive lymphocytes were obtained from 22 donor mice, which were actively immunized as previously described (31, 34) using 200 mg of the myelin peptide PLP139-151 emulsified with 200 ml complete Freund´s adjuvant (Thermo Fischer Scientific, United States) and 800 mg Mycobacterium tuberculosis
H37Ra (Difico, United States). As described in (31), on days 0 and 2 postimmunization (p.i.), 250 ng pertussis toxin (List Biological Laboratories, United States) was injected intraperitoneally. Donor animals were sacrificed on day 10 p.i., axillary and inguinal lymph nodes were collected and cells cultured as described (35). Cells were harvested after 4 days in culture with RPMI 1640 medium (supplemented with 2 mM L-glutamine, 100 units/ml penicillin, 100 mg/ml streptomycin and 10 % fetal bovine serum) (Gibco, Thermo Fischer Scientific) containing 12.5 mg/ml PLP. Into each of the 21 recipient mice 30 million cells were intraperitoneally injected. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)