Bacterial Toxin Research Citations

EAE:

…Immediately following MOG peptide injection and 24 h later mice were intraperitoneally (ip) administered 200 µg pertussis toxin (List Biological Labs, Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and clinical assessment of EAE:

C57BL/6J Jcl (B6) female mice (n = 5-6 per group, 7-8 weeks old) were immunized subcutaneously at the base of the tail with 100 µg of myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) peptide (amino acid sequence, MEVGWYRSPFSRVVHYRNGK, derived from mouse MOG) dissolved in 0.1 ml phosphate buffered saline (PBS) and 0.1 ml CFA containing 1 mg of Mycobacterium tuberculosis strain H37Ra (Mtb H37Ra). Shortly after immunization and 48 h later, the mice were injected i.p. with 200 ng of Bordetella pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

C57BL/6J mice were injected s.c. with 50 µg MOG p3555 or 100 µg rmMOG or 100 µg rhMOG emulsified in CFA (DIFCO Laboratories) containing 200 µg heat-killed Mycobacterium tuberculosis (Mtb) H37RA (DIFCO Laboratories) on day 0. Additionally, mice received i.v. 200 ng Bordetella pertussis toxin (List Biological Laboratories) in 0.2 ml PBS on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cytotoxicity studies:  Recombinant B. anthracis lethal factor was purchased from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Lethal Factor was utilized.  List Labs offers Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

… STZ is a widely used and accepted model of type 1 diabetes despite its nonspecific tissue toxicity (8, 9). DT or mutant DT (List Biological Laboratories) was injected intraperitoneally at the dose of 25 ng/g body wt weekly for 6 wk beginning 1 wk after STZ injection. …

• Product #149A – Diphtheria Toxin CRM197, Mutant
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… We injected mice intraperitoneally with … purified B. pertussis toxin (List Biologicals) on days 0, 3, and 7 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… C57BL/6J mice received 200 ng pertussis toxin (List Biological Laboratories, Hornby, ON, Canada) in 500 µl sterile phosphate-buffered saline (PBS) on the day of immunization and 48 h later. Clinical symptoms …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Ex vivo activation of peripheral blood mononuclear cells:

…For the activation of monocytes, 200 l PBMC were stimulated in separate wells (ninety-six-well plates) with 10 g/l of ultra-pure lipopolysaccharide (LPS; List Biological Laboratories, Inc.) or an equal volume of sterile endotoxin-free water as a negative control. PBMC were incubated at 37C in 5 % CO₂ and supernatants were removed after 24, 48 and 72 h for the analysis of monocyte cytokine production as described below.

Author has informed that the following LPS was utilized:

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Mice:

Tumors that were 69 mm in diameter were selected, and volumes were monitored by high-resolution ultrasound as detailed in… KPC (DTR transgene) mice were treated every 48 h with 25 ng/g of DTx (List Biologicals) in PBS,…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

… Cholera toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

[32P]ADP-ribosylation Assays
ADP-ribosylation was performed essentially as described (33), with minor modifications. Briefly, cholera toxin was activated at a concentration of 2 mg/ml by incubation for 30 min at 30 °C in media containing 100 mm DTT, 10 mg/ml BSA, 1% SDS, and 200 mm HEPES, pH 7.5. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae