Bacterial Toxin Research Citations

Experimental Protocol:

Autoimmune cholangitis was induced as previously described …injected intraperitoneally (I.P.) into 8 week-old female B6 mice. The mice were subsequently boosted 2 weeks later with 2OA-BSA in incomplete Freunds adjuvant (IFA, Sigma-Aldrich). Additionally, mice received 100 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) on the same day and 2 days following the initial …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… They were also injected with 500 ng of pertussis toxin (List Biological Laboratories, Inc, Campbell, CA) intraperitoneally on Day 0 and 2. 20 µg of cholera toxin (List Biological Laboratories, Inc, Campbell, CA)…100 ng/ml of LPS (List Biological Laboratories) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Bordetella pertussis toxin (200 ng, PTX, List Biological Laboratories, Campbell, CA) was administered intra-peritoneally at days 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAE:

C57BL/6, trkB+/ and trkB+/+ (wt littermates) mice (5 generations of backcrossing, JAX stock 002544) were immunized subcutaneously on day zero in four sites over the back (left and right shoulder, left and right flank) with 200 g of MOG₃₅₅₅ (MEVGWYRSPFSRVVHLYRNGK) (Princeton Biomolecules, Langhorne, PA) emulsified in a volume of 100 l of complete Freunds adjuvant (CFA) containing 1 mg/ml of M. tuberculosis (M. Tbc) (CFA, Difco Laboratories, Detroit, MI, USA) (ONeill et al., 2006; Papenfuss et al., 2004). On days 0 and 2 post-immunization, 200 ng of pertussis toxin (PT) (List Biological, Campbell, CA) was administered intraperitoneally (i.p.) in 0.2 ml of PBS …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

IMC isolation and differentiation:

B6 mice were immunized with an emulsion of 100 ml PBS and 100 ml CFA… Each animal also received 200 ng pertussis toxin (PT; List Biological Laboratories) i.v. on days 0 and 2 postimmunization. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental protocol:

…To examine the role of chemoattractant receptors and CCR4 on Treg migration during CS, mice underwent sensitization and challenge as already described. Subsequently, anesthetized mice were injected intradermally with either PTx (List Biological Laboratories, Campbell, CA; 100 ng in PBS)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE exacerbation, pharmacokinetics and treatment:

C57BL/6 mice were immunized subcutaneously at four sites in the flanks with 100 µg hMOG3555 peptide… 200 ng of pertussis toxin (List Biological Laboratories) was injected i.p. on days 0 and 2 to disrupt the blood-brain barrier. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Cell Line and Cell Culture:

… After mice were killed, the eyeballs were enucleated by the pathogen-free disposable punch (2.5 mm), and washed with Ca²⁺-free PBS (pH 7.2) containing 1X antibiotic-antimycotic… After 7 to 10 days, nearly 100% confluence of the cells formed, the half corneal button could be placed in a new cell culture plate to start a new explant culture as described above. …and subcultured at CnT-50 containing 100 ng/mL cholera toxin (LIST Biological Laboratories, Inc., Campbell, CA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell culture and treatments:

… 10 ng/mL cholera toxin (List Biological Laboratories, Inc.), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… LPS was obtained from List Biological Laboratories (San Jose, CA, USA) and was dissolved in endotoxin-free water. …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/