Bacterial Toxin Research Citations

… Reagents and cell culture:

Staphylococcal enterotoxin B was obtained from List Biological Laboratories Inc. (Campbell, CA, USA). …

Reagents:

LPS (Escherichia coli serotype O111:B4) was purchased from List Biological Laboratories, INC (Campbell, CA); …

• Product #201 – LPS from Escherichia coli O111:B4

Induction, treatment, and clinical evaluation of EAE:

The murine EAE model was produced as described previously [56]. Briefly, female C57BL/6 mice were immunized on day 0 by subcutaneous (s.c.) injection with 150 µl of an emulsion containning MOG₃₅₅₅ CFA …Each mouse received an additional 400 ng of Bordetella pertussis toxin (List Biological Laboratory) by intraperitoneal (i.p.) injection in 200 µl of PBS on day 0 and day 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Anatomy:

A separate set of tissue containing NTS coming from the same group of treated rats (n = 4) was incubated overnight at room temperature with the primary antibody for CTb (1:20,000, Goat anti-CTb; 703, List Biological Laboratories)

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

C57BL/6 mice received 200 ng per mouse pertussis toxin (List Biological Laboratories Inc.) i.p. on the day of immunization and 2 d thereafter.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pharmacological Treatments:

To ablate AgRP neurons in adult mice, we administered two i.m. injections of DT (50 mg/kg; List Biological Laboratories) 2 d apart to 6-wk-old mice…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Administration of CT:

Mice were i.g. inoculated under anesthesia (as described above) with 10 µg CT (List Biological Laboratories, Campbell, CA) in a volume of 50 µl of 8.5% NaHCO₃ 16 to 24 h prior to infection. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Toxin challenge:

An in vivo oral CT challenge test was used as described previously (Tokuhara et al., 2010). After being fasted for 12 h, mice (10 per group) were orally challenged with 20 µg of CT (List Biological Laboratories, Campbell, CA)….

GM1-binding inhibition assay:

A GM1-binding inhibition assay was performed using a GM1-ELISA as described previously, with some modifications…The serum of macaques (10%, v/v) was treated with CT at a final concentration of 50 ng/mL for 1 h at RT and then incubated in 96-well plates…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents:

…LPS from Escherichia coli O111:B4 was purchased from List Biological Laboratories Inc. (Campbell, CA, USA). …

Results:

BCR stimulation elicits transcriptional and post-transcriptional activation of IB-:

Induction of IB- mRNA in LPS-stimulated macrophages requires NF-B-mediated transcriptional activation of IB- (10, 11). We, therefore, analyzed activation of the IB- promoter by reporter analyses. Upon stimulation of A20 cells with F(ab)₂ of anti-immunoglobulin antibody, a reporter harboring an IB- promoter fragment from 1725 to +115bp was significantly activated similar to the NF-B reporter pELAM1-Luc (Fig. 2A). A further truncated IB- promoter from 786bp was also activated in response to BCR engagement (Fig. 2B). This promoter region contains three NF-B binding sites, B1, B2 and B3. Introduction of mutations at the B2 site severely impaired activation in response to BCR engagement as in LPS-stimulated macrophages (11), indicating a critical role for NF-B in the transcriptional activation. Mutations at the B1 site moderately reduced activation, but mutations at the B3 site, whose sequence is not conserved between mouse and human, did not affect activation.

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

…An intraperitoneal injection of 300 ng pertussis toxin (List Biological Labs, Campbell, CA, USA) was administered on the day of induction and 48 h later in EAE immunized mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)