Bacterial Toxin Research Citations

… After centrifugation at 10,000 rpm for 60 min at 4 °C, the supernatant was filter-sterilized using distilled and deionized water, and lyophilized at 70 °C. Cholera toxin (CT) was purchased from List Biological Laboratories, Inc. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… J774A.1 macrophage-like cells were cultured in DMEM supplemented with 10% FBS (Sigma) and 2 mM l-glutamine. Cells were incubated with 11000 ng/ml LPS (ultra pure from E. coli O111:B4, List Biological Labs) at 37 C in DMEM/10% FBS. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

SH-SY5Y cell culture, and cell treatment with TLR agonists, with Ca^2 +/ionomycin, and with TLR pathways inhibitors:

… The TLR agonists used were: … lipopolysaccharide LPS (E. coli K12, D31m4(Re)) (List Biological Laboratories Inc., California, USA) (stock solution of 1 mg/ml) was added at a final concentration of 100 ng/ml; …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)

Bactericidal assay:

… LPS competition was carried out as described previously (8) by preincubating sera or plasma for 30 to 60 min at room temperature with 100 g/ml of purified LPS from either S. Typhimurium or Escherichia coli (O111:B4) before they were tested for bactericidal activity. Both LPS preparations were obtained from List Biological Laboratories, Inc., Campbell, CA (catalog number 225 or 201, respectively). …

• Product #225 – LPS from Salmonella typhimurium
• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

LPS was obtained from List Biological Laboratories (San Jose, CA, USA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

RNAi experiments:

RNAi using RAW264.7 cells was performed largely as described [43]. … Twenty-four hours after siRNA transfection, cells were stimulated with the indicated PAMPs for six hours. Doses used were 20 ng/ml LPS (List Biological Labs), …

Author indicated that the following LPS product was used:

• Product #201 – LPS from Escherichia coli O111:B4

Reagents:

… LPS from E. coli O111:B4 for in vitro experiments was purchased from List Biological Laboratories; …

Immunoprecipitations and Western blotting analysis:

HEK 293T cells were transfected with plasmids encoding 4-1BBL and TLR4, and cell lysates were prepared and incubated with the appropriate primary antibodies before being incubated with Protein G-agarose. The immunoprecipitates were then resolved by SDS-PAGE and analyzed by Western blotting according to standard protocols. To examine physical interactions between endogenous proteins, we treated mouse peritoneal macrophages with LPS, subjected cell lysates to immunoprecipitation with anti-TLR4 or anti-TIRAP antibodies, and then analyzed samples by Western blotting, which was performed with chemiluminescence substrate, and bands corresponding to proteins of interest were visualized with x-ray film or with the Odyssey scanner (LI-COR Bioscience, Lincoln, NE).

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

…Immediately following the MOG 33-55 injection, mice were injected ip with 300 µl of salt-free pertussis toxin (List Biological Laboratories, INC, Campbell, CA at 1 ng/l) on the same day and again 48 h later.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

Active EAE was induced using previously-described protocols [79]. In accordance with Canadian Council on Animal Care (CCAC) and UBC guidelines, female 6-8 week old CL57BL/6 mice … 200 ng Pertussis toxin (List Biologicals, Campbell, CA) was delivered intraperitoneally at immunization and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Control animals were administered CFA without PLP peptide. Two and 48 h after EAE induction, all animals received an intraperitoneal injection of 400 ng of pertussis toxin (List Biological Laboratories, Campbell, Calif., USA) in 100 l of saline. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)