Bacterial Toxin Research Citations

EAE:

…Mice received 20 ng of pertussis toxin (PTX; List Biological Laboratories) intravenously at the time of immunization and 48 hr later. For passive-transfer EAE induction, C57Bl/6J WT mice were injected with 20 ng PTX 4 days and 1 day before cell transfer.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… Purified pertussis toxin (PTX) was purchased from List Biological Laboratories (Campbell, CA). …

Induction and Scoring of EAU:

Mice were immunized by injection of 0.2 mL emulsion containing 40 µg bovine whole IRBP in CFA, administered by subcutaneous injections into the tail base and the two thighs. PTX, 0.2 µg, was injected intraperitoneally, concurrently with the immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2. EAE immunization:

C57Bl/6 mice were immunized subcutaneously with 200 µg of MOG₃₅₅₅ … Pertussis toxin (List Biological Laboratories, Campbell, CA) (250 ng) was injected i.p. on the day of immunization and 2 days later. Mice were monitored daily for clinical signs of disease and were scored as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and treatment of EAE:

Active induction of EAE involved s.c. injection of 100 µg MOG₃₅₅₅ MEVGWYRSP-FSRVVHLYRNGK… Each mouse also received 300 ng of Bordetella PT(List Biological Laboratories) i.p. on day 0 and 2 postimmunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

Additionally, the mice received 100 ng of pertussis toxin (List Biological Laboratories, Inc.) i.p. on days 0 and 2. Mice were monitored daily and scored for disease severity using the standard scale…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE, treatment with THI, immune cell proliferation and cytokine analysis

EAE was induced in 8-9 weeks-old female WT or S1P₁(S5A) mice (n = 10 per group) immunized with Complete Freunds Adjuvant (CFA) and Myelin Oligoglycoprotein (MOG) peptide₃₅₅₅ and Bordetella pertussis toxin (List Biological Laboratories) 400 ng per mouse on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Dorsal column primary sensory axon tracing:

Dorsal column sensory projections from the sciatic nerve to the nucleus gracilis in the medualla were traced with choleratoxin B (CTB)… Two µl of 1% cholera toxin B (CTB; List Biologicals, Campbell CA) solution was then slowly injected over 1 minute and the needle left in place for an additional 2 minutes. The needle was then withdrawn from the nerve and the ligature removed. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental Materials:

Stx2 toxin was purchased from List Biological Laboratories, Inc. (Lot #1621A1, Campbell, CA). Endotoxin levels were tested by List Biological Laboratories and found to be acceptable. …

Determination of Mean Lethal Dose:

Groups of at least 10 randomly selected mice were treated by intraperitoneal (ip) injection with 500 L per dose of serial dilutions of Stx2 (in a range that spans high lethality to no deaths). …

Toxicokinetics of Stx2:

The biologic half-lives of Stx2 were determined in the presence or absence of mAbs against Stx2. Mice were treated iv with 100 ng per mouse (100 L of 1,000 ng/mL stock) of Stx2. …

Treatment of Mice Post-intoxication or Pre-intoxication with Stx2 mAbs:

To simulate post-intoxication treatment, mice were treated by iv with 100 L of 180 ng/mL of Stx2. …

List Labs has discontinued Product #162 (Lyophilized) and has replaced Product #162 with Product #162L (Liquid):

• Product #162L (Liquid) – Shiga Toxin 2 from Escherichia coli

…Lipopolysaccharide (LPS E. coli serotype 011:B4) was purchased from List Biological Laboratories (Campbell, CA).  …

Determination of TLR4 Ligand Presence in BAL Fluid and Serum:

…All conditions were tested in duplicate in the presence or absence of polymixin B (50 g/ml), which binds to LPS and prevents recognition by TLR4. Positive control wells were treated with LPS at 10 ng/ml with or without polymixin B. Negative control wells were treated with medium only.

• Product #201 – LPS from Escherichia coli O111:B4

Materials and Methods – Stx and monoclonal antibodies (mAbs):

Pure Stx1 was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

Results – Development and characterization of a polyclonal antibody against Stx2:

…The pAb obtained bound to both the recombinant Stx2a toxoid and the wild type Stx2a but did not react with Stx1 when tested by direct ELISA (Table 3), indicating the pAb is Stx2-specific. …

• Product #161 – Shiga Toxin 1 from Escherichia coli