Bacterial Toxin Research Citations

In vitro mast cellactivation assay:

For activation assays, 2.5 3 105 BMCMCs were plated/well in a 96-well U-bottom plate. Virus was added at a multiplicity of infection (MOI) 1, and the nal volume was brought to 100 ml and incubated for 46 h. As positive controls, BMCMCs were stimulated with LPS (5 mg/ml; List Biologicals Laboratory) for cytokine/chemokine release or the calcium ionophore A23187…

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

… microglial cells were plated per well in 12-well plates in DMEM supplemented as above but in 2% FBS, and was left to settle for 24 h. Thereafter, the medium was changed to DMEM without serum, and with or without 100 nM lipopolysaccharide (LPS) (List biological lab). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Isolation and stimulation of neonatal rat CFB:

…Following 24 h starvation from serum, the cells were stimulated for 24 h with vehicle (sterile NaCl/Pi), 10 ngmL 1 IL-1b (PRC0814; Gibco), 25 ngmL 1 IL-6 (406-ML; R&D Systems, Minneapolis, MN, USA), 1 lgmL 1 LPS (201; List Biological Laboratories, Campell, CA, USA) or …

• Product #201 – LPS from Escherichia coli O111:B4

Primary cultures of neonatal cardiac myocytes and fibroblasts:

…Following 24 h serum starvation, isolated neonatal cardiomyocytes and fibroblasts were stimulated with 10 ngmL¹ IL-1 (PRC0814; Gibco, Camarillo, CA, USA), 50 ngmL¹ TNF (PMC3014; Gibco), 1 gmL¹ LPS (201; List Biological Laboratories, Campell, CA, USA), …

• Product #201 – LPS from Escherichia coli O111:B4

EAE induction:

EAE was induced by active immunization with an emulsion made with MOG35-55
and Mycobacterium tuberculosis. … Each mouse received 0.05
mL of emulsion subcutaneously at four sites.  When immunized for clinical disease
course, mice also received 300 ng pertussis toxin (PT, List Biological Labs Inc.,
Campbel, CA) prepared in with phosphate buffered saline (PBS) to keep the disease onset
consistent throughout the mice.  The PT/PBS mixture was injected intraperitoneally at 0
and 72 hours post immunization, per standard laboratory protocol.  

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Pertussis toxin (500 ng, List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally on the day of the immunization and again 2 days later, as described. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE in DR2-Tg:

… HLA-DR2-positive male and female mice between 8 and 12 weeks of age were immunized s.c. at four sites on the flanks with 0.2 mL of an emulsion of 200 g immunogenic peptide and complete Freund’s adjuvant… In addition, mice were given pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA, USA) on days 0 and 2 post immunization (75 and 200 ng per mouse, respectively). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization:

… To induce development of EAE, the MOG-peptideimmunized B6 mice were also injected twice (on the day of immunization and 2 days later) with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) in PBS.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

EAE was induced by s.c. immunization of mice into the flanks with an emulsion of MOG3555 peptide (100 mg) emulsified in CFA supplemented with 4 mg/ml Mycobacterium tuberculosis extract H37Ra (Difco). Additionally, the animals received 200 ng pertussis toxin (List Biological Laboratories) i.p. on days 0 and 2. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

On Days 0 and 2, animals received ip injections of 250 ng pertussis toxin (List Biologicals.)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)