Citations

Animals and induction of experimental autoimmune encephalomyelitis:

Female SJL/J mice were purchased from Harlan Laboratories (Borchen, Germany) and C57BL/6 J mice were purchased from Janvier (Le Genest Saint Isle, France). For the induction of EAE, female mice at age eight weeks, received subcutaneous injections in both flanks of either 50 µg MOG_35-55 peptide or 100 µg PLP_139-151 peptide … Two injections of pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA; 200 ng per mouse intraperitoneal) were given on the day of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents and cell lines:

The following reagents were used: LPS from Escherichia coli O111:B4 (List Biological Laboratories), …

• Product #201 – LPS from Escherichia coli O111:B4

EAE Induction:

… Mice also received two i.p. injections with 200 ng of pertussis toxin (List Biologicals) at the time of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and Scoring of EAE:

EAE was actively induced by subcutaneous administration of 200 µL suspension at 2 sites over the hind flanks. The suspension consisted of 300 µg MOG₃₅₅₅ peptide (Sigma Aldrich, Germany) in 100 µL of PBS, emulsified with 100 µL complete Freund`s adjuvant (Sigma Aldrich, Germany) with 0.7 mg heat-inactivated Mycobacterium tuberculosis (strain H37 RA; Difco Laboratories, Detroit, MI). Each mouse was immediately thereafter, injected intraperitonealy and 48 hours later with 300 ng pertussis toxin (List Biological Laboratories, Campbell, USA) in 100 µL 0.9% NaCl. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… and Bordetella pertussis toxin (Ptx) was obtained from List Biological (Campbell, CA). …

Induction, Treatment and Scoring of EAE:

Six to eight week old female SJL/J mice were immunized s.c. with 200 µg (PLP)₁₃₉₁₅₁ peptide emulsified in CFA in the rear flank. The mice also received 325 ng of Ptx i.p. immediately after immunization and again on day 2. Immunized mice received vehicle or 150 nmol/mouse 1V136 s.c. daily from day 6 to day 18. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

Female wild-type (WT) C57BL/6 or pik3cg^/ were immunized subcutaneously in the hind flanks and at the scruff of the neck with a total of 25 µg Myelin Oligodendrocyte Glycoprotein (MOG) peptide-₃₅₅₅ … Two hours prior to immunization, and two days after, the mice were intravenously injected with 300 ng of pertussis toxin (List Biological Laboratories Inc., California, USA) in endotoxin-free PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

… Pertussis toxin from List biological laboratories, Cat. No. 179A (Campbell, CA), …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Vaccine formulations:

The vaccines discussed in this report were composed of rPA (List Biological Laboratories), recombinant spore-specific proteins produced as described earlier ( Brahmbhatt et al., 2007 ; Cybulski et al., 2008 ; Mikesell et al., 1983 ) or a combination of the antigens. The specific amounts of antigens used in the vaccine formulations are described in the figure legend of each experiment. Aluminium hydroxide gel (AL; aluminium content per vaccination ~125 g for mice or ~500 g for guinea pigs) or the Sigma adjuvant system (SAS) was used as adjuvant (Sigma Aldrich). SAS is an emulsion of monophosphoryl lipid A and trehalose dicorynomycolate, which may promote mixed T helper 1 (Th1)/Th2 immune responses to vaccines, and was used as directed by the manufacturer.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… Cholera toxin and insulin were from List Biological (Campbell, CA, USA) and Invitrogen (Carlsbad, CA, USA), respectively. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

… SNAPtide(o-Abz-Dnp) was purchased from List Biological Laboratory. …

SNAPtide based LC activity assay:

SNAPtide (o-Abz-Dnp) (List Biological Laboratories) was used for studying the LCs
activity and inhibition (Figure 2.2). 100 M SNAPtide stock was prepared in DMSO.
The proteolytic reaction was initiated by the addition of LC to 20 mM HEPES (pH 7.5)
with 2 M SNAPtide. The protease activity was monitored by the increase of o-Abz
fluorescence (ex: 320 nm, em: 420 nm). The results were analyzed using Sigmaplot. …

• Product #520 –  SNAPtide Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin